Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways

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	Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways
	Yan, AF; Chen, YF; Chen, S; Li, SS; Zhang, Y; Jia, JR; Yu, H; Liu, L; Liu, F; Hu, CQ
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2018
卷号	18 期号:12 页码:-
通讯作者	yanaifen@mail3.sysu.edu.cn ; chyfwf@hotmail.com ; chenss@connect.hku.hk ; lshuish@mail.sysu.edu.cn ; lsszy@mail.sysu.edu.cn ; jiajr@mail2.sysu.edu.cn ; yu71hui@126.com ; lian2004@163.com ; 1115fang@sina.com ; hucq@scsio.ac.cn ; tangdsh@163.com ; chan1010@scsio.ac.cn
英文摘要	Leptin actions at the pituitary level have been extensively investigated in mammalian species, but remain insufficiently characterized in lower vertebrates, especially in teleost fish. Prolactin (PRL) is a pituitary hormone of central importance to osmoregulation in fish. Using goldfish as a model, we examined the global and brain-pituitary distribution of a leptin receptor (lepR) and examined the relationship between expression of lepR and major pituitary hormones in different pituitary regions. The effects of recombinant goldfish leptin-AI and leptin-AII on PRL mRNA expression in the pituitary were further analysed, and the mechanisms underlying signal transduction for leptin-induced PRL expression were determined by pharmacological approaches. Our results showed that goldfish lepR is abundantly expressed in the brain-pituitary regions, with highly overlapping PRL transcripts within the pituitary. Recombinant goldfish leptin-AI and leptin-AII proteins could stimulate PRL mRNA expression in dose-and time-dependent manners in the goldfish pituitary, by both intraperitoneal injection and primary cell incubation approaches. Moreover, the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK, and MEK1/2/ERK1/2-but not JAK2/STAT 1, 3 and 5 cascades-were involved in leptin-induced PRL mRNA expression in the goldfish pituitary.
学科主题	Biochemistry & Molecular Biology; Chemistry
关键词[WOS]	goldfish ; leptin ; prolactin ; pituitary ; expression regulation ; signal pathway
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/17039]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Yan, AF,Chen, YF,Chen, S,et al. Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,18(12):-.
APA	Yan, AF.,Chen, YF.,Chen, S.,Li, SS.,Zhang, Y.,...&Chen, T.(2018).Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(12),-.
MLA	Yan, AF,et al."Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.12(2018):-.