Cytotoxic Anthracycline Metabolites from a Recombinant Streptomyces

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	Cytotoxic Anthracycline Metabolites from a Recombinant Streptomyces
	Zhang, SW; Huang, HB; Mo, XH; Ju, JH; Yuan, J; Gu, YC; Gui, C
刊名	JOURNAL OF NATURAL PRODUCTS
	2018
卷号	81 期号:5 页码:1278-1289
通讯作者	jju@scsio.ac.cn
英文摘要	The C7 (C9 or C10)-O-L-rhodosamine-bearing anthracydine antibiotic cytorhodins and their biosynthetic intermediates were recently isolated from Streptomyces sp. SCSIO 1666. Cosmid p17C4 from the Streptomyces lydicus genomic library, which harbors both the biosynthetic genes for L-rhodinose (or 2-deoxy-L-fucose) and its glycosyltransferase (encoded by slgG), was introduced into SCSIO 1666 to yield the recombinant strain Streptomyces sp. SCSIO 1666/17C4. Chemical investigations of this strain's secondary metabolic potential revealed the production of different anthracyclines featuring C7-O-L-rhodinose (or 2-deoxy-L-fucose) instead of the typically observed L-rhodosamine. Purification of the fermentation broth yielded 12 new anthracycline antibiotics including three new epsilon-rhodomycinone derivatives, 1, 4, and 8, nine new beta-rhodomycinone derivatives, 2, 3, 5-7, and 9-12, three known compounds, L-rhodinose-L-rhodinose-L-rhodinoserhodomycinone (13), e-rhodomycinone (14), and gamma-rhodomycinone (15). All compounds were characterized on the basis of detailed spectroscopic analyses and comparisons with previously reported data. These compounds exhibited cytotoxicity against a panel of human cancer cell lines. Significantly, compounds 4 and 13 displayed pronounced activity against HCT-116 as characterized by IC50 values of 0.3 and 0.2 mu M, respectively; these IC50 values are comparable to that of the positive control epirubicin.
学科主题	Plant Sciences; Pharmacology & Pharmacy
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/16957]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Zhang, SW,Huang, HB,Mo, XH,et al. Cytotoxic Anthracycline Metabolites from a Recombinant Streptomyces[J]. JOURNAL OF NATURAL PRODUCTS,2018,81(5):1278-1289.
APA	Zhang, SW.,Huang, HB.,Mo, XH.,Ju, JH.,Yuan, J.,...&Gui, C.(2018).Cytotoxic Anthracycline Metabolites from a Recombinant Streptomyces.JOURNAL OF NATURAL PRODUCTS,81(5),1278-1289.
MLA	Zhang, SW,et al."Cytotoxic Anthracycline Metabolites from a Recombinant Streptomyces".JOURNAL OF NATURAL PRODUCTS 81.5(2018):1278-1289.