Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level

doi:10.1016/j.bios.2017.08.017

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院合肥智能机械研究所

	Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level
	Chen, Xiangyu 1,2; Liang, Zhiting 1; Li, Daobo 3; Xiong, Ying 1; Xiong, Penghui 1; Guan, Yong 1; Hou, Shuangyue 1; Hu, Yue 1; Chen, Shan 1; Liu, Gang1
刊名	BIOSENSORS & BIOELECTRONICS
	2018-01-15
卷号	99 期号:无页码:416-423
关键词	Microfluidic Chip Dielectrophoresis Shewanella Oneidensis Fluorescence Cell Level
DOI	10.1016/j.bios.2017.08.017
文献子类	Article
英文摘要	Shewanella oneidensis, a model organism for electrochemical activity bacteria, has been widely studied at the biofilm level. However, to obtain more information regarding this species, it is essential to develop an approach to trap and detect S. oneidensis at the cell level. In this study, we report a rapid and label-free microfluidic platform for trapping, counting and detecting S. oneidensis cells. A microfluidic chip was integrated with a modified dielectrophoresis (DEP) trapping technique and hole arrays of different hole sizes. By numerical simulation and an elaborate electric field distribution design, S. oneidensis cells were successfully trapped and positioned in the hole arrays. Real time fluorescence imaging was also used to observe the trapping process. With the aid of a homemade image program, the trapped bacteria were accurately counted, and the results demonstrated that the amount of bacteria correlated with the hole sizes. As one of the significant applications of the device, Raman identification and detection of countable S. oneidensis cells was accomplished in two kinds of holes. The microfluidic platform provides a quantitative sample preparation and analysis method at the cell level that could be widely applied in the environmental and energy fields.
WOS关键词	LABEL-FREE DETECTION ; ESCHERICHIA-COLI ; TECHNOLOGY ; MR-1 ; BIODECOLORIZATION ; SEPARATION ; BACTERIA ; WATER ; NOV
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000413284200056
资助机构	National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Key Basic Research Program of China(2016YFA0400902) ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; National Science Foundation of China(11275204 ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; Dean Foundation of Hefei Institute of Physical Science, CAS(YZJJ201514) ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11475175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 11405175 ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123) ; 21507123)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/33813]
专题	合肥物质科学研究院_中科院合肥智能机械研究所
作者单位	1.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiangyu,Liang, Zhiting,Li, Daobo,et al. Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level[J]. BIOSENSORS & BIOELECTRONICS,2018,99(无):416-423.
APA	Chen, Xiangyu.,Liang, Zhiting.,Li, Daobo.,Xiong, Ying.,Xiong, Penghui.,...&Tian, Yangchao.(2018).Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level.BIOSENSORS & BIOELECTRONICS,99(无),416-423.
MLA	Chen, Xiangyu,et al."Microfluidic dielectrophoresis device for trapping, counting and detecting Shewanella oneidensis at the cell level".BIOSENSORS & BIOELECTRONICS 99.无(2018):416-423.