Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

doi:10.1039/c7lc00053g

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip
	Zhai, Zhimin 1,2; Zhang, Fengqiu 1,2,3; Chen, Xiangyu 2; Zhong, Jie 1,2; Liu, Gang2 ; Tian, Yangchao 2; Huang, Qing 1,2
刊名	LAB ON A CHIP
	2017-04-07
卷号	17 期号:7 页码:1306-1313
DOI	10.1039/c7lc00053g
文献子类	Article
英文摘要	This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS). The nanoparticles were fabricated as beta-cyclodextrin-coated silver nanoparticles (Ag@CD NPs) modified with para-aminothiophenol (p-ATP) and folic acid (FA) on the surface. The p-ATP molecules act as the Raman reporter while the FA tags have high affinity for folate receptors (FR) that are over-expressed on the surface cancerous cells, so that the nanoparticles can enter the cells and be monitored by the Raman reporter. Therefore, the nanoparticles could be utilized not only as cell invaders due to endocytosis but also as a SERS sensitive probe to monitor the effect of FR-targeted drugs such as dihydroartemisinin (DHA) that induce the population change of FR on the membrane of living cells. As a result, we have successfully demonstrated that we are able to employ the Ag@CD@p-ATP@FA NPs to evaluate the number of NPs entering living cells quantitatively and correspondingly the drug effect on cancer cells in a well-controlled way.
WOS关键词	LABEL-FREE DETECTION ; FOLATE RECEPTOR EXPRESSION ; GOLD NANOPARTICLES ; FOLIC-ACID ; SCATTERING SPECTROSCOPY ; MOLECULAR RECOGNITION ; SERS NANOPARTICLES ; OVARIAN-CANCER ; SINGLE-CELL ; IN-VIVO
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000398955800010
资助机构	National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Natural Science Foundation of China(11635013) ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; National Basic Research Program of China(2013CB934304 ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002) ; 2014CB932002)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/33345]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Zhengzhou Univ, Sch Phys Engn, Zhengzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhai, Zhimin,Zhang, Fengqiu,Chen, Xiangyu,et al. Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip[J]. LAB ON A CHIP,2017,17(7):1306-1313.
APA	Zhai, Zhimin.,Zhang, Fengqiu.,Chen, Xiangyu.,Zhong, Jie.,Liu, Gang.,...&Huang, Qing.(2017).Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip.LAB ON A CHIP,17(7),1306-1313.
MLA	Zhai, Zhimin,et al."Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip".LAB ON A CHIP 17.7(2017):1306-1313.