A sustainable biogenic route to synthesize quantum dots with tunable fluorescence properties for live cell imaging
Tian, Li-Jiao1; Zhou, Nan-Qing1; Liu, Xian-Wei1; Liu, Jing-Hua2; Zhang, Xing1; Huang, Hai1; Zhu, Ting-Ting3; Li, Ling-Li1; Huang, Qing2; Li, Wen-Wei1
刊名BIOCHEMICAL ENGINEERING JOURNAL
2017-08-15
卷号124期号:页码:130-137
关键词Quantum Dots (Qds) Cdse Sustainable Tunable Biosynthesis Fluorescence Properties
DOI10.1016/j.bej.2017.05.011
文献子类Article
英文摘要Quantum dots (QDs), owning to their unique optical and electronic properties, are increasingly used for bio-imaging/biosensing in medical and energy fields. Biosynthesis of QDs offers several advantages over the conventional chemical synthesis route, such as low cost, environmental-benignity, and no need for further modification of the products. However, information about the regulation and mechanism of synthesis of QDs with great fluorescence properties is still limited. In this work, we prepared cadmium selenium (CdSe) QDs in vivo using Candida utilis WSH02-08. We regulated the fluorescence properties of the bio-QDs through different precursor concentrations, and found that the bio-QDs with high fluorescence intensity and long photostable lifetime were favoured at an elevated Cd content over Se. The synthesis of the high-quality bio-QDs was attributed to the less formation of Se(0) and the increased formation of CdSe and CdS. Furthermore, such synthesized QDs were directly used in live-cell imaging without further modification. These findings are expected to enable a further design of biosynthesized QDs with great fluorescence properties and expand our understanding of the regulation mechanism of nanoparticles fabrication in microorganisms. (C) 2017 Elsevier B.V. All rights reserved.
WOS关键词ESCHERICHIA-COLI ; YEAST-CELLS ; BIOSYNTHESIS ; NANOCRYSTALS ; GLUTATHIONE ; NANOPARTICLES ; SPECTROSCOPY ; DIAGNOSTICS
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
语种英语
WOS记录号WOS:000404199100016
资助机构Natural Science Foundation of China(21477120 ; Natural Science Foundation of China(21477120 ; Natural Science Foundation of China(21477120 ; Natural Science Foundation of China(21477120 ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; 51538011) ; 51538011) ; 51538011) ; 51538011) ; Natural Science Foundation of China(21477120 ; Natural Science Foundation of China(21477120 ; Natural Science Foundation of China(21477120 ; Natural Science Foundation of China(21477120 ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Program for Changjiang Scholars and Innovative Research Team in University ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; Collaborative Innovation Center of Suzhou Nano Science and Technology of the Ministry of Education of China ; 51538011) ; 51538011) ; 51538011) ; 51538011)
内容类型期刊论文
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/31994]  
专题合肥物质科学研究院_技术生物与农业工程研究所
作者单位1.Dept Chem, CAS Key Lab Urban Pollutant Convers, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol, Hefei 230031, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714
Tian, Li-Jiao,Zhou, Nan-Qing,Liu, Xian-Wei,et al. A sustainable biogenic route to synthesize quantum dots with tunable fluorescence properties for live cell imaging[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,124(无):130-137.
APA Tian, Li-Jiao.,Zhou, Nan-Qing.,Liu, Xian-Wei.,Liu, Jing-Hua.,Zhang, Xing.,...&Yu, Han-Qing.(2017).A sustainable biogenic route to synthesize quantum dots with tunable fluorescence properties for live cell imaging.BIOCHEMICAL ENGINEERING JOURNAL,124(无),130-137.
MLA Tian, Li-Jiao,et al."A sustainable biogenic route to synthesize quantum dots with tunable fluorescence properties for live cell imaging".BIOCHEMICAL ENGINEERING JOURNAL 124.无(2017):130-137.
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