A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide

doi:10.1016/j.cplett.2016.05.055

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	A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide
	Ke, Zhigang 1; Ma, Shanshan 1; Li, Lamei 1; Huang, Qing 1,2
刊名	CHEMICAL PHYSICS LETTERS
	2016-07-16
卷号	657 期号:无页码:49-52
关键词	Fluorescence Horseradish Peroxidase Protein Unfolding Heme
DOI	10.1016/j.cplett.2016.05.055
文献子类	Article
英文摘要	Horseradish peroxidase (HRP) is a classical heme-containing protein which has been applied in many fields. The prosthetic group heme in HRP, especially in unfolded state, can react with hydrogen peroxide (H2O2) to produce a fluorescent product with the maximum emission wavelength at 450 nm. Utilizing this emission band as a fluorescence probe, the unfolding process of HRP in urea can be assessed quantitatively, and the calculated thermodynamic parameters are consistent with those determined by circular dichroism (CD) at 222 nm and steady-state tryptophan (Trp) fluorescence methods. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	NUCLEAR-MAGNETIC-RESONANCE ; X-RAY-SCATTERING ; CIRCULAR-DICHROISM ; GUANIDINE-HYDROCHLORIDE ; CYTOCHROME-C ; PROTEINS ; DENATURATION ; SPECTROSCOPY ; INACTIVATION ; STABILITY
WOS研究方向	Chemistry ; Physics
语种	英语
WOS记录号	WOS:000383444700009
资助机构	Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; 11175204 ; 11175204 ; 11175204 ; 11175204 ; 11475217 ; 11475217 ; 11475217 ; 11475217 ; 11405220) ; 11405220) ; 11405220) ; 11405220) ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Natural Science Foundation of China(10975152 ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; Youth Innovation Promotion Association of the Chinese Academy of Sciences(2014289) ; 11175204 ; 11175204 ; 11175204 ; 11175204 ; 11475217 ; 11475217 ; 11475217 ; 11475217 ; 11405220) ; 11405220) ; 11405220) ; 11405220)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/24255]
专题	合肥物质科学研究院_技术生物与农业工程研究所
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Ke, Zhigang,Ma, Shanshan,Li, Lamei,et al. A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide[J]. CHEMICAL PHYSICS LETTERS,2016,657(无):49-52.
APA	Ke, Zhigang,Ma, Shanshan,Li, Lamei,&Huang, Qing.(2016).A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide.CHEMICAL PHYSICS LETTERS,657(无),49-52.
MLA	Ke, Zhigang,et al."A fluorescence approach to the unfolding thermodynamics of horseradish peroxidase based on heme degradation by hydrogen peroxide".CHEMICAL PHYSICS LETTERS 657.无(2016):49-52.