Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica

doi:10.1016/j.algal.2017.03.004

	Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica
	Yao, Changhong 1; Meng, Yingying 1,2; Yang, Qing 2; Cao, Xupeng 1; Xue, Song 1
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2017-06-01
卷号	24 页码:122-129
关键词	Nannochloropsis Lipidomics Polar Glycerolipids Metabolism Tag Accumulation Nitrogen Starvation
DOI	10.1016/j.algal.2017.03.004
英文摘要	Nannochloropsis, an ocean microalgal genus, is a promising resource of biodiesel feedstock because of its capacity for triacylglycerol (TAG) accumulation. Polar glycerolipids have big contribution for TAG accumulation by providing acyl group or diacylglycerol (DAG). Due to the diversities and amount of polar glycerolipids in species, a suitable approach has not been developed well to quantify and qualify, which veils the clarification of how important roles the polar glycerolipids play in microalgal lipid metabolism and TAG accumulation. In this study, a total of 112 polar glycerolipid species, covering eight classes, were identified using UPLC/Q-TRAP plus Orbitrap coupled with quantified using UPLC/Q-TOF. A comprehensive profiling of polar glycerolipid was established to study the polar glycerolipids variations in response to nitrogen deprivation in Nannochloropsis oceanica. Through targeted lipidomics analysis it was proposed that in N. oceanica, phosphatidylcholine (PC) serve as linoleic and gamma-linolenic acyl donors via oleic acyl desaturation, and under nitrogen stress condition PC transfers acyl 16:0 and 18:1 to TAG. The result disclosed PC played import roles for TAG accumulation not only in high plants but also in PC-containing microalgae even PC is not the most extraplastidial lipid. In addition, it was showed that phosphatidylethanolamine (PE) and diacylglyceryl-N-trimethylhomoserine (DGTS) are suppliers of eicosapentaenoic acid (EPA) to other lipids. These new discoveries reveal the diversity of microalgae in acyl metabolism and shed light on the understanding of lipid biosynthesis by stoichiometry. (C) 2017 Published by Elsevier B.V.
语种	英语
WOS记录号	WOS:000404864600012
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/152077]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
推荐引用方式 GB/T 7714	Yao, Changhong,Meng, Yingying,Yang, Qing,et al. Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2017,24:122-129.
APA	Yao, Changhong,Meng, Yingying,Yang, Qing,Cao, Xupeng,&Xue, Song.(2017).Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,24,122-129.
MLA	Yao, Changhong,et al."Identification of the role of polar glycerolipids in lipid metabolism and their acyl attribution for TAG accumulation in Nannochloropsis oceanica".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 24(2017):122-129.