AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77

doi:10.3390/molecules22122259

	AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77
	Zhang, Bo 1,4; Zong, Yuan 2; Ye, Guangji 2; Cao, Dong 1,3,4; Zhang, Huaigang1,4 ; Liu, Baolong 1,4; Chen, Wenjie 1,4
刊名	MOLECULES
	2017-12-01
卷号	22 期号:12
关键词	Ae Tauschii Coleoptile Anthocyanin Biosynthesis Bhlh Transcription Factor Myb Transcription Factor
DOI	10.3390/molecules22122259
英文摘要	The red coleoptile trait can help monocotyledonous plants withstand stresses, and key genes responsible for the trait have been isolated from Triticum aestivum, Triticum urartu, and Triticum monococcum, but no corresponding research has been reported for Aegilops tauschii. In this research, transcriptome analysis was performed to isolate the candidate gene controlling the white coleoptile trait in Ae. tauschii. There were 5348 upregulated, differentially-expressed genes (DEGs) and 4761 downregulated DEGs in red coleoptile vs. white coleoptile plants. Among these DEGs, 12 structural genes and two transcription factors involved in anthocyanin biosynthesis were identified. The majority of structural genes showed lower transcript abundance in the white coleoptile of accession As77' than in the red coleoptile of accession As60', which implied that transcription factors related to anthocyanin biosynthesis could be the candidate genes. The MYB and MYC transcription factors AetMYB7D and AetMYC1 were both isolated from Ae. tauschii accessions As60' and As77', and their transcript levels analyzed. The coding sequence and transcript level of AetMYB7D showed no difference between As60' and As77'. AetMYC1p encoded a 567-amino acid polypeptide in As60' containing the entire characteristic domains, bHLH-MYC_N, HLH, and ACT-like, belonging to the gene family involved in regulating anthocyanin biosynthesis. AetMYC1w encoded a 436-amino acid polypeptide in As77' without the ACT-like domain because a single nucleotide mutation at 1310 bp caused premature termination. Transient expression of AetMYC1p induced anthocyanin biosynthesis in As77' with the co-expression of AetMYB7D, while AetMYC1w could not cause induced anthocyanin biosynthesis under the same circumstances. Moreover, the transcript abundance of AetMYC1w was lower than that of AetMYC1p. AetMYC1 appears to be the candidate gene controlling the white coleoptile trait in Ae. tauschii, which can be used for potential biotech applications, such as producing new synthetic hexaploid wheat lines with different coleoptile colors.
语种	英语
WOS记录号	WOS:000419242400221
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13295]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Qinghai Prov Key Lab Crop Mol Breeding, Xining 810008, Qinghai, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 800010, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Northwest Inst Plateau Biol, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bo,Zong, Yuan,Ye, Guangji,et al. AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77[J]. MOLECULES,2017,22(12).
APA	Zhang, Bo.,Zong, Yuan.,Ye, Guangji.,Cao, Dong.,Zhang, Huaigang.,...&Chen, Wenjie.(2017).AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77.MOLECULES,22(12).
MLA	Zhang, Bo,et al."AetMYC1, the Candidate Gene Controlling the Red Coleoptile Trait in Aegilops tauschii Coss. Accession As77".MOLECULES 22.12(2017).