Binding constant of cell adhesion receptors and substrate-immobilized ligands depends on the distribution of ligands

doi:10.1103/PhysRevE.97.012405

CORC > 力学研究所 > 中国科学院力学研究所 > 非线性力学国家重点实验室

	Binding constant of cell adhesion receptors and substrate-immobilized ligands depends on the distribution of ligands
	Li L(李龙); Hu JL; Xu GK ; Song F(宋凡)
刊名	PHYSICAL REVIEW E
	2018-01-16
卷号	97 期号:1 页码:12405
ISSN号	2470-0045
DOI	10.1103/PhysRevE.97.012405
英文摘要	Cell-cell adhesion and the adhesion of cells to tissues and extracellular matrix, which are pivotal for immune response, tissue development, and cell locomotion, depend sensitively on the binding constant of receptor and ligand molecules anchored on the apposing surfaces. An important question remains of whether the immobilization of ligands affects the affinity of binding with cell adhesion receptors. We have investigated the adhesion of multicomponent membranes to a flat substrate coated with immobile ligands using Monte Carlo simulations of a statistical mesoscopic model with biologically relevant parameters. We find that the binding of the adhesion receptors to ligands immobilized on the substrate is strongly affected by the ligand distribution. In the case of ligand clusters, the receptor-ligand binding constant can be significantly enhanced due to the less translational entropy loss of lipid-raft domains in the model cell membranes upon the formation of additional complexes. For ligands randomly or uniformly immobilized on the substrate, the binding constant is rather decreased since the receptors localized in lipid-raft domains have to pay an energetic penalty in order to bind ligands. Our findings help to understand why cell-substrate adhesion experiments for measuring the impact of lipid rafts on the receptor-ligand interactions led to contradictory results.
分类号	一类
URL标识	查看原文
WOS关键词	MEMBRANE-ANCHORED RECEPTORS ; LIPID RAFTS ; PLASMA-MEMBRANE ; L-SELECTIN ; KINETICS ; TCR ; FLOW ; FLUCTUATIONS ; MIGRATION ; RESPONSES
WOS研究方向	Physics, Fluids & Plasmas ; Physics, Mathematical
语种	英语
WOS记录号	WOS:000423129500013
资助机构	Programs in the National Natural Science Foundation of China [11472285, 11232013, 21504038, 11402193] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB22040102] ; National Key Research and Development Program of China [2016YFA0501601] ; Fundamental Research Funds for the Central Universities ; Shenzhen Science and Technology Innovation Committee [JCYJ20160531151105346]
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/77792]
专题	力学研究所_非线性力学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China 3.Nanjing Univ, Kuang Yaming Honors Sch, Nanjing 210023, Jiangsu, Peoples R China 4.Nanjing Univ, Shenzhen Inst Res, Shenzhen 518057, Peoples R China 5.Xi An Jiao Tong Univ, Sch Aerosp, Xian 710049, Shaanxi, Peoples R China 6.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li L,Hu JL,Xu GK,et al. Binding constant of cell adhesion receptors and substrate-immobilized ligands depends on the distribution of ligands[J]. PHYSICAL REVIEW E,2018,97(1):12405.
APA	李龙,Hu JL,Xu GK,&宋凡.(2018).Binding constant of cell adhesion receptors and substrate-immobilized ligands depends on the distribution of ligands.PHYSICAL REVIEW E,97(1),12405.
MLA	李龙,et al."Binding constant of cell adhesion receptors and substrate-immobilized ligands depends on the distribution of ligands".PHYSICAL REVIEW E 97.1(2018):12405.