Crystal Structure of the GRAS Domain of SCARECROW-LIKE7 in Oryza sativa

doi:10.1105/tpc.16.00018

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Crystal Structure of the GRAS Domain of SCARECROW-LIKE7 in Oryza sativa
	Li, Shengping 1; Zhao, Yanhe 1; Zhao, Zheng 2; Wu, Xiuling 1; Sun, Lifang 1; Liu, Qingsong 3; Wu, Yunkun 1
刊名	PLANT CELL
	2016-05-01
卷号	28 期号:5 页码:1025-1034
DOI	10.1105/tpc.16.00018
文献子类	Article
英文摘要	GRAS proteins belong to a plant-specific protein family with many members and play essential roles in plant growth and development, functioning primarily in transcriptional regulation. Proteins in the family are minimally defined as containing the conserved GRAS domain. Here, we determined the structure of the GRAS domain of Os-SCL7 from rice (Oryza sativa) to 1.82 angstrom. The structure includes cap and core subdomains and elucidates the features of the conserved GRAS LRI, VHIID, LRII, PFYRE, and SAW motifs. The structure is a dimer, with a clear groove to accommodate double-stranded DNA. Docking a DNA segment into the groove to generate an Os-SCL7/DNA complex provides insight into the DNA binding mechanism of GRAS proteins. Furthermore, the in vitro DNA binding property of Os-SCL7 and model-defined recognition residues are assessed by electrophoretic mobility shift analysis and mutagenesis assays. These studies reveal the structure and preliminary DNA interaction mechanisms of GRAS proteins and open the door to in-depth investigation and understanding of the individual pathways in which they play important roles.
WOS关键词	SIGNAL-TRANSDUCTION ; ARABIDOPSIS ROOT ; TRANSCRIPTION FACTOR ; GENE-EXPRESSION ; PROTEIN FAMILY ; DNA-BINDING ; GIBBERELLIN ; RESPONSES ; REGULATOR ; MECHANISM
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种	英语
WOS记录号	WOS:000378080000008
资助机构	National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Nature Science Foundation of China(31270790 ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; National Thousand Talents Program of China ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31470741 ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218) ; 31500218)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22453]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China 2.NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA 3.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Li, Shengping,Zhao, Yanhe,Zhao, Zheng,et al. Crystal Structure of the GRAS Domain of SCARECROW-LIKE7 in Oryza sativa[J]. PLANT CELL,2016,28(5):1025-1034.
APA	Li, Shengping.,Zhao, Yanhe.,Zhao, Zheng.,Wu, Xiuling.,Sun, Lifang.,...&Wu, Yunkun.(2016).Crystal Structure of the GRAS Domain of SCARECROW-LIKE7 in Oryza sativa.PLANT CELL,28(5),1025-1034.
MLA	Li, Shengping,et al."Crystal Structure of the GRAS Domain of SCARECROW-LIKE7 in Oryza sativa".PLANT CELL 28.5(2016):1025-1034.