Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification

doi:10.1021/jacs.6b00515

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification
	Zheng, Ji-Shen 1,2; He, Yao 1,2; Zuo, Chao 3; Cai, Xiao-Ying 1,2; Tang, Shan 3; Wang, Zhipeng A.3; Zhang, Long-Hua 1,2; Tian, Chang-Lin 1,2; Liu, Lei 3
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2016-03-16
卷号	138 期号:10 页码:3553-3561
DOI	10.1021/jacs.6b00515
文献子类	Article
英文摘要	Chemical protein synthesis can provide access to proteins with post-translational modifications or site-specific labelings. Although this technology is finding increasing applications in the studies of water-soluble globular proteins, chemical synthesis of membrane proteins remains elusive. In this report, a general and robust removable backbone modification (RBM) method is developed for the chemical synthesis of membrane proteins. This method uses an activated O-to-N acyl transfer auxiliary to install in the Fmoc solid-phase peptide synthesis process a RBM group with switchable reactivity toward trifluoroacetic acid. The method can be applied to versatile membrane proteins because the RBM group can be placed at any primary amino acid. With RBM, the membrane proteins and their segments behave almost as if they were water-soluble peptides and can be easily handled in the process of ligation, purification, and mass characterizations. After the full-length protein is assembled, the RBM group can be readily removed by trifluoroacetic acid. The efficiency and usefulness of the new method has been demonstrated by the successful synthesis of a two-transmembrane-domain protein (HCV p7 ion channel) with site-specific isotopic labeling and a four-transmembrane-domain protein (multidrug resistance transporter EmrE). This method enables practical synthesis of small- to medium-sized membrane proteins or membrane protein domains for biochemical and biophysical studies.
WOS关键词	HEPATITIS-C VIRUS ; X-RAY-STRUCTURE ; MULTIDRUG TRANSPORTER ; TRANSMEMBRANE DOMAIN ; ESCHERICHIA-COLI ; NMR-SPECTROSCOPY ; ION-CHANNEL ; BIOLOGICAL FUNCTIONS ; DIFFICULT PEPTIDES ; PROTON CHANNEL
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000372477700042
资助机构	NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; NSFC(21532004 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; MOST(2015CB910100 ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; CAS(XDB08030302) ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 21225207 ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 2013CB932800) ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; 21402206 ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138) ; U1332138)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22336]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230031, Peoples R China 3.Tsinghua Univ, Dept Chem, Tsinghua Peking Ctr Life Sci, Key Lab Bioorgan Phosphorus Chem Chem Biol,Minist, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Ji-Shen,He, Yao,Zuo, Chao,et al. Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2016,138(10):3553-3561.
APA	Zheng, Ji-Shen.,He, Yao.,Zuo, Chao.,Cai, Xiao-Ying.,Tang, Shan.,...&Liu, Lei.(2016).Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,138(10),3553-3561.
MLA	Zheng, Ji-Shen,et al."Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 138.10(2016):3553-3561.