A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems

	A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems
	Yang, Ling 2; Qian, Xing-Kai 1; Wang, Ping 1; Zou, Li-Wei 1,2; Yu, Yang 1; Wang, Dan-Dan 1; Jin, Qiang 1; Hou, Jie 3; Liu, Zhi-Hong 4; Ge, Guang-Bo 1
刊名	BIOSENSORS & BIOELECTRONICS
	2017-04-15
卷号	90 页码:283-289
关键词	Dipeptidyl peptidase IV Two-photon fluorescent probe Cells and tissues imaging Inhibitor screening
英文摘要	In this study, a highly specific ratiometric two-photon fluorescent probe GP-BAN was developed and well characterized to monitor dipeptidyl peptidase IV in plasma and living systems. GP-BAN was designed on the basis of the catalytic properties and substrate preference of DPP-IV, and it could be readily hydrolyzed upon addition of DPP-IV under physiological conditions. Both reaction phenotyping and inhibition assays demonstrated that GP-BAN displayed good reactivity and high selectivity towards DPP-IV over other human serine hydrolases including FAP, DPP-VIII, and DPP-IX. The probe was successfully used to monitor the real activities of DPP-IV in complex biological systems including diluted plasma, while it could be used for high throughput screening of DPP-W inhibitors by using human plasma or tissue preparations as enzyme sources. As a two photon fluorescent probe, GP-BAN was also successfully used for two-photon imaging of endogenous DPP-IV in living cells and tissues, and showed high ratiometric imaging resolution and deep-tissue penetration ability. Taken together, a ratiometric two-photon fluorescent probe GP-BAN was developed and well-characterized for highly selective and sensitive detection of DPP-IV in complex biological systems, which could serve as a promising imaging tool to explore the biological functions and physiological roles of this key enzyme in living systems.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]	FIBROBLAST ACTIVATION PROTEIN ; HUMAN CARBOXYLESTERASE 2 ; IN-VITRO ; BIOLOGICAL APPLICATIONS ; CANCER ; CELLS ; CD26 ; ASSOCIATION ; MICROSCOPY ; INHIBITORS
收录类别	SCI
语种	英语
WOS记录号	WOS:000392768000039
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/151895]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China 3.Dalian Med Univ, Dalian 116044, Peoples R China 4.Wuhan Univ, Key Lab Analyt Chem Biol & Med, Minist Educ, Coll Chem & Mol Sci, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Yang, Ling,Qian, Xing-Kai,Wang, Ping,et al. A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems[J]. BIOSENSORS & BIOELECTRONICS,2017,90:283-289.
APA	Yang, Ling.,Qian, Xing-Kai.,Wang, Ping.,Zou, Li-Wei.,Yu, Yang.,...&Feng, Lei.(2017).A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems.BIOSENSORS & BIOELECTRONICS,90,283-289.
MLA	Yang, Ling,et al."A highly specific ratiometric two-photon fluorescent probe to detect dipeptidyl peptidase IV in plasma and living systems".BIOSENSORS & BIOELECTRONICS 90(2017):283-289.