Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides

	Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides
	Qin, Hongqiang 1; Wu, Ren'an 1; Peng, Jiaxi 1,3; Zhang, Hongyan 1,3; Li, Xin 1,3; Liu, Shengju 1,3; Zhao, Xingyun 1,3; Wu, Jing 4; Kang, Xiaohui 1; Pan, Zaifa 2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2016-12-28
卷号	8 期号:51 页码:35012-35020
关键词	metal-affinity probe immobilized metal ion affinity metal oxide affinity metal-organic framework phosphorylated peptide
英文摘要	The highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium(W) on a zirconium organic framework of UiO-66-NH2, which holds dual metal centers of not only the inherent Zr-O cluster but also the immobilized Zr(IV) center. This dual-metal centered zirconium-organic framework (DZMOF) demonstrates as a highly specific metal-affinity probe toward the extraction of phosphopeptides due to the metal-affinity interactions of MOAC and IMAC toward either mono-phosphorylated or multi-phosphorylated peptides. The binding energies of zirconium 3d(5/2) and 3d(3/2) in this DZMOF are 183.07 and 185.47 eV, respectively, which are higher than those of the intact UiO-66-NH2 (182.84 and 185.17 eV, respectively), confirming the higher metal-affinity interaction between the DZMOF and phosphopeptides. This high metal-affinity probe presents an unprecedented strong performance in anti nonspecific interference during the capturing of phosphopeptides of beta-casein with the molar ratio of beta-casein vs bovine serum albumin up to ca. 1:5000. The enrichment of phosphopeptides from a human saliva sample by DZMOF further confirms the great potential of DZMOF in the extraction of low-abundance phosphopeptides for real complex biological samples.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]	Science & Technology - Other Topics ; Materials Science
关键词[WOS]	MASS-SPECTROMETRIC ANALYSIS ; SELECTIVE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PHOSPHORYLATED PEPTIDES ; FACILE SYNTHESIS ; MAGNETIC NANOPARTICLES ; SIGNALING NETWORKS ; COMPLEX-MIXTURES ; MICROSPHERES ; CHROMATOGRAPHY
收录类别	SCI
语种	英语
WOS记录号	WOS:000391081700009
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/151845]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Zhejiang, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Wenzhou Inst Biomat & Engn, Wenzhou 325000, Peoples R China
推荐引用方式 GB/T 7714	Qin, Hongqiang,Wu, Ren'an,Peng, Jiaxi,et al. Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(51):35012-35020.
APA	Qin, Hongqiang.,Wu, Ren'an.,Peng, Jiaxi.,Zhang, Hongyan.,Li, Xin.,...&Pan, Zaifa.(2016).Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides.ACS APPLIED MATERIALS & INTERFACES,8(51),35012-35020.
MLA	Qin, Hongqiang,et al."Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides".ACS APPLIED MATERIALS & INTERFACES 8.51(2016):35012-35020.