Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation

	Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation
	Wu, Jianqiang 3; Song, Juan 1,3; Liu, Hui 3; Zhuang, Huifu 1,3; Zhao, Chunxia 4; Xu, Yuxing 1,3; Wu, Shibo 2; Qi, Jinfeng 3; Li, Jing 3; Hettenhausen, Christian 3
刊名	FRONTIERS IN PLANT SCIENCE
	2017-10-10
卷号	8
关键词	maize (Zea mays L.) Rhopalosiphum padi transcriptome alternative splicing metabolites benzoxazinoid
英文摘要	Maize (Zea mays L.) is a staple crop worldwide with extensive genetic variations. Various insects attack maize plants causing large yield loss. Here, we investigated the responses of maize B73, a susceptible line, and Mo17, a resistant line, to the aphid Rhopalosiphum padi on metabolite and transcriptome levels. R. padi feeding had no effect on the levels of the defensive metabolites benzoxazinoids (Bxs) in either line, and Mo17 contained substantially greater levels of Bxs than did B73. Profiling of the differentially expressed genes revealed that B73 and Mo17 responded to R. padi infestation specifically, and importantly, these two lines showed large gene expression differences even without R. padi herbivory. Correlation analysis identified four transcription factors (TFs) that might account for the high Bx levels in Mo17. Similarly, genome-wide alternative splicing (AS) analyses indicated that both B73 and Mo17 had temporally specific responses to R. padi infestation, and these two lines also exhibited large differences of AS regulation under normal condition, and 340 genes, including 10 TFs, were constantly differentially spliced. This study provides large-scale resource datasets for further studies on the mechanisms underlying maize-aphid interactions, and highlights the phenotypic divergence in defense against aphids among maize varieties.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	CORN LEAF APHID ; ARABIDOPSIS-THALIANA ; NICOTIANA-ATTENUATA ; PHENOLIC-COMPOUNDS ; DEFENSE RESPONSES ; INSECT HERBIVORES ; HYDROXAMIC ACIDS ; ORAL SECRETIONS ; MYZUS-PERSICAE ; CEREAL APHIDS
收录类别	SCI
语种	英语
WOS记录号	WOS:000412627200001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150187]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Yunnan Acad Sci & Technol Dev, Kunming, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming, Yunnan, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Wu, Jianqiang,Song, Juan,Liu, Hui,et al. Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Wu, Jianqiang.,Song, Juan.,Liu, Hui.,Zhuang, Huifu.,Zhao, Chunxia.,...&Hettenhausen, Christian.(2017).Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation.FRONTIERS IN PLANT SCIENCE,8.
MLA	Wu, Jianqiang,et al."Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation".FRONTIERS IN PLANT SCIENCE 8(2017).