Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS

	Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS
	Wang, Qi 1,2; Wang, Keyun 1,2; Ye, Mingliang 1,2
刊名	ANALYST
	2017-10-17
卷号	142 期号:19 页码:3536-3548
英文摘要	Protein methylation is an important post-translational modification (PTM) that plays crucial roles in the regulation of diverse biological processes. Though many efforts have been devoted to the investigation of protein methylation, the analysis of non-histone methylation at the proteome level is still a great challenge. The alteration of the protein/peptide physicochemical properties caused by methylation is very small, thus it is difficult to develop highly efficient enrichment approaches to separate methylated peptides from a pool of diverse background peptides. The mass shifts caused by methylations are identical to the substitutions of some amino acids, thus it is difficult to confidently identify methylated peptides. In this review, we report on recent advances in the development of methods for large-scale analysis of nonhistone protein methylation. Especially the methods for efficient enrichment and the approaches for controlling identification confidence have been covered.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	ADENOSYL-L-METHIONINE ; AFFINITY-CHROMATOGRAPHY ADSORBENT ; POSITIVE-ION MODE ; MASS-SPECTROMETRY ; LYSINE-METHYLATION ; ARGININE METHYLATION ; PROTEOMIC ANALYSIS ; POSTTRANSLATIONAL MODIFICATIONS ; HUMAN-CELLS ; IN-VIVO
收录类别	SCI
语种	英语
WOS记录号	WOS:000411703800001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150048]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qi,Wang, Keyun,Ye, Mingliang. Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS[J]. ANALYST,2017,142(19):3536-3548.
APA	Wang, Qi,Wang, Keyun,&Ye, Mingliang.(2017).Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS.ANALYST,142(19),3536-3548.
MLA	Wang, Qi,et al."Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS".ANALYST 142.19(2017):3536-3548.