ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo

	ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo
	Yin, Shanye 5; Wang, Lantian 3; He, Zhisong 1; Wu, Xudong 2; Zhang, Heng 3; Shi, Min 3; Cheng, Hong 3; Li, Guohui 2; Tian, Bin 4
刊名	NUCLEIC ACIDS RESEARCH
	2017-09-19
卷号	45 期号:16 页码:9640-9653
英文摘要	The TREX complex (TREX) plays key roles in nuclear export of mRNAs. However, little is known about its transcriptome-wide binding targets. We used individual cross-linking and immunoprecipitation (iCLIP) to identify the binding sites of ALYREF, an mRNA export adaptor in TREX, in human cells. Consistent with previous in vitro studies, ALYREF binds to a region near the 5' end of the mRNA in a CBP80-dependent manner. Unexpectedly, we identified PABPN1-dependent ALYREF binding near the 3' end of the mRNA. Furthermore, the 3' processing factor CstF64 directly interacts with ALYREF and is required for the overall binding of ALYREF on the mRNA. In addition, we found that numerous middle exons harbor ALYREF binding sites and identified ALYREF-binding motifs that promote nuclear export of intronless mRNAs. Together, our study defines enrichment of ALYREF binding sites at the 5' and the 3' regions of the mRNA in vivo, identifies export-promoting ALYREF-binding motifs, and reveals CstF64-and PABPN1-mediated coupling of mRNA nuclear export to 3' processing.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	HUMAN TREX COMPLEX ; GENE-EXPRESSION ; NUCLEAR EXPORT ; COTRANSCRIPTIONAL RECRUITMENT ; SPLICING PROMOTES ; MRNP FORMATION ; THO COMPLEX ; INTRONLESS ; ELEMENT ; TRANSPORT
收录类别	SCI
语种	英语
WOS记录号	WOS:000411096400037
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150044]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China 4.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA 5.Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA
推荐引用方式 GB/T 7714	Yin, Shanye,Wang, Lantian,He, Zhisong,et al. ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo[J]. NUCLEIC ACIDS RESEARCH,2017,45(16):9640-9653.
APA	Yin, Shanye.,Wang, Lantian.,He, Zhisong.,Wu, Xudong.,Zhang, Heng.,...&Tian, Bin.(2017).ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo.NUCLEIC ACIDS RESEARCH,45(16),9640-9653.
MLA	Yin, Shanye,et al."ALYREF mainly binds to the 5 ' and the 3 ' regions of the mRNA in vivo".NUCLEIC ACIDS RESEARCH 45.16(2017):9640-9653.