The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide

	The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide
	Xu, Qingsong 1,2; Liu, Meisi 1; Liu, Qishun 1; Wang, Wenxia 1; Du, Yuguang 3; Yin, Heng 1
刊名	CARBOHYDRATE POLYMERS
	2017-10-15
卷号	174 页码:1138-1143
关键词	N-acetylated chitooligosaccharide Lipopolysaccharides Interleukin-6 Tumor necrosis factor-alpha Inflammation PI3K/Akt pathway
英文摘要	Chitooligosaccharide (COS) has been shown to regulate many biological functions, such as antimicrobial effect and antitumor activity. In the present study, highly N-acetylated chitooligosaccharide(NACOS) was prepared by N-acetylation of COS, and the anti-inflammatory activity of NACOS in macrophages were evaluated. The results indicated NACOS significantly suppressed the LPS-induced proinflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha(TNF-alpha) expression. Furthermore, the increased levels of reactive oxygen species (ROS) and nitric oxide (NO) were repressed by NACOS in a dose dependent manner. However, NACOS itself had no significant effect on the cell viability and cellular morphology. Signal transduction studies demonstrated that NACOS remarkably inhibited LPS-enhanced phosphorylation of phosphatidylinositol 3-kinase (PI3K) and Akt. These findings provide a possible molecular mechanism by which NACOS inhibit LPS-induced inflammatory response in macrophages, and a basis for utilizing NACOS in pharmaceutical therapy against inflammation. (C) 2017 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	RAW 264.7 CELLS ; CHITOSAN OLIGOSACCHARIDES ; IN-VITRO ; ENDOTHELIAL-CELLS ; MOLECULAR-WEIGHT ; CHITIN ; EXPRESSION ; MICROGLIA ; DISEASES ; VIVO
收录类别	SCI
语种	英语
WOS记录号	WOS:000407696800119
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149906]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xu, Qingsong,Liu, Meisi,Liu, Qishun,et al. The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide[J]. CARBOHYDRATE POLYMERS,2017,174:1138-1143.
APA	Xu, Qingsong,Liu, Meisi,Liu, Qishun,Wang, Wenxia,Du, Yuguang,&Yin, Heng.(2017).The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide.CARBOHYDRATE POLYMERS,174,1138-1143.
MLA	Xu, Qingsong,et al."The inhibition of LPS-induced inflammation in RAW264.7 macrophages via the PI3K/Akt pathway by highly N-acetylated chitooligosaccharide".CARBOHYDRATE POLYMERS 174(2017):1138-1143.