Epigenetic silencing of microRNA-137 enhances ASCT2 expression and tumor glutamine metabolism | |
Dong, J.2,3; Xiao, D.3; Zhao, Z.4; Ren, P.5; Li, C.6; Hu, Y.3; Shi, J.7; Su, H.3,7; Wang, L.3; Liu, H.3 | |
刊名 | ONCOGENESIS |
2017-07-01 | |
卷号 | 6 |
英文摘要 | Tumor cells must activate specific transporters to meet their increased glutamine metabolic demands. Relative to other glutamine transporters, the ASC family transporter 2 (ASCT2, also called SLC1A5) is profoundly elevated in a wide spectrum of human cancers to coordinate metabolic reprogramming and malignant transformation. Understanding the molecular mechanisms whereby tumor cells frequently upregulate this transporter is therefore vital to develop potential strategies for transporter-targeted therapies. Combining in-silico algorithms with systemic experimental screening, we herein identify the tumor suppressor microRNA, miR-137, as an essential regulator that targets ASCT2 and cancer cell glutamine metabolism. Metabolic analysis shows that miR-137 derepression, similar to ASCT2 inactivation, significantly inhibits glutamine consumption and TCA cycle anaplerosis. Mechanistically, methyl-CpG-binding protein 2 (MeCP2) and DNA methyltransferases (DNMTs) cooperate to promote active methylation of the miR-137 promoter and inhibit its transcription, conversely reactivating ASCT2 expression and glutamine metabolism. Moreover, expression between miR-137 and ASCT2 is inversely correlated in tumor specimens from multiple cancer types, and ectopic ASCT2 expression markedly rescued miR-137 suppression of tumorigenesis. These findings thus elucidate a previously unreported mechanism responsible for ASCT2 deregulation in human cancers and identify ASCT2 as a critical downstream effector of miR-137, revealing a molecular link between DNA methylation, microRNA and tumor metabolism. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Oncology |
研究领域[WOS] | Oncology |
关键词[WOS] | MAMMALIAN-CELLS ; CANCER GROWTH ; MYC ; NEUROBLASTOMA ; MIR-137 ; SUPPRESSION ; ACTIVATION ; CARBOXYLATION ; TRANSPORTERS ; DEPRIVATION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000408140600008 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/149880] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China 2.Huazhong Univ Sci & Technol, Sch Basic Med, Dept Pharmacol, Tongji Med Coll, Wuhan, Hubei, Peoples R China 3.Wuhan Univ, Med Res Inst, Dept Canc Biol, 185 East Lake Rd, Wuhan 430071, Hubei, Peoples R China 4.Dalian Med Univ, Clin Lab, Hosp 2, Dalian, Peoples R China 5.Hubei Univ Sci & Technol, Sch Pharm, Xianning, Peoples R China 6.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Biochem & Mol Biol, Guangzhou, Guangdong, Peoples R China 7.Huazhong Univ Sicence & Technol, Union Hosp, Tongji Med Coll, Wuhan, Hubei, Peoples R China 8.Dalian Med Univ, Affiliated Dalian Peoples Hosp 6, Dalian, Peoples R China |
推荐引用方式 GB/T 7714 | Dong, J.,Xiao, D.,Zhao, Z.,et al. Epigenetic silencing of microRNA-137 enhances ASCT2 expression and tumor glutamine metabolism[J]. ONCOGENESIS,2017,6. |
APA | Dong, J..,Xiao, D..,Zhao, Z..,Ren, P..,Li, C..,...&Qing, G..(2017).Epigenetic silencing of microRNA-137 enhances ASCT2 expression and tumor glutamine metabolism.ONCOGENESIS,6. |
MLA | Dong, J.,et al."Epigenetic silencing of microRNA-137 enhances ASCT2 expression and tumor glutamine metabolism".ONCOGENESIS 6(2017). |
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