Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells

	Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells
	Xu, Cong 1,2; Wang, Li 1,2; Yu, Yue 1,2; Yin, Fangchao 1,2; Zhang, Xiaoqing 1; Jiang, Lei 1,2; Qin, Jianhua 1,2
刊名	BIOMATERIALS SCIENCE
	2017-09-01
卷号	5 期号:9 页码:1810-1819
英文摘要	Organized cardiomyocyte alignment is critical to maintain the mechanical properties of the heart. In this study, we present a new and simple strategy to fabricate a biomimetic microchip designed with an onion epithelium-like structure and investigate the guided behavior of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) on the substrate. The hiPSC-CMs were observed to be confined by the three dimensional surficial features morphologically, analogous to the in vivo microenvironment, and exhibited an organized anisotropic alignment on the onion epithelium-like structure with good beating function. The calcium imaging of hiPSC-CMs demonstrated a more mature Ca2+ spark pattern as well. Furthermore, the expression of sarcomere genes (TNNI3, MYH6 and MYH7), potassium channel genes (KCNE1 and KCNH2), and calcium channel genes (RYR2) was significantly up-regulated on the substrate with an onion epithelium-like structure instead of the surface without the structure, indicating a more matured status of cardiomyocytes induced by this structure. It appears that the biomimetic micro-patterned structure, analogous to in vivo cellular organization, is an important factor that might promote the maturation of hiPSC-CMs, providing new biological insights to guide hiPSC-CM maturation by biophysical factors. The established approach may offer an effective in vitro model for investigating cardiomyocyte differentiation, maturation and tissue engineering applications.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Biomaterials
研究领域[WOS]	Materials Science
关键词[WOS]	FUNCTIONAL MATURATION ; HUMAN MYOCARDIUM ; CULTURE MODEL ; BETA-CATENIN ; HEART ; GENERATION ; CONTRACTILITY ; GROWTH ; CHIP ; CUES
收录类别	SCI
语种	英语
WOS记录号	WOS:000408253000011
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149878]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xu, Cong,Wang, Li,Yu, Yue,et al. Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells[J]. BIOMATERIALS SCIENCE,2017,5(9):1810-1819.
APA	Xu, Cong.,Wang, Li.,Yu, Yue.,Yin, Fangchao.,Zhang, Xiaoqing.,...&Qin, Jianhua.(2017).Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells.BIOMATERIALS SCIENCE,5(9),1810-1819.
MLA	Xu, Cong,et al."Bioinspired onion epithelium-like structure promotes the maturation of cardiomyocytes derived from human pluripotent stem cells".BIOMATERIALS SCIENCE 5.9(2017):1810-1819.