Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides

	Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides
	Qian, Xiaohong 3; Jiao, Fenglong 2,3; Gao, Fangyuan 3; Wang, Heping 3; Deng, Yulin 2; Zhang, Yangjun 3; Zhang, Yukui 1
刊名	SCIENTIFIC REPORTS
	2017-08-01
卷号	7
英文摘要	The low abundance of glycopeptides in biological samples makes it necessary to enrich them before further analysis. In this study, the polymeric hydrophilic ionic liquid-modified magnetic (Fe3O4@MPS@PMAC) nanoparticles were synthesized via a one-step reflux-precipitation polymerization. Owing to the excellent hydrophilicity and strong electrostatic interaction toward glycopeptides of the polymerized hydrophilic ionic liquid, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (MAC), the synthesized Fe3O4@MPS@PMAC nanoparticles exhibited outstanding performance in glycopeptide enrichment with high detection sensitivity (10 fmol), large binding capacity (100 mu g mg(-1)) and satisfied enrichment recovery (approximately 82%). Furthermore, the newly developed Fe3O4@MPS@PMAC nanoparticles were applied for the glycopeptide enrichment of HeLa exosome proteins. A total of 1274 glycopeptides from 536 glycoproteins were identified in three replicate analyses of 50 mu g of HeLa exosome proteins. These results demonstrate the potential of Fe3O4@MPS@PMAC nanoparticles for both glycoproteomic analysis and exosome research.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	PROTEIN GLYCOSYLATION ; EFFICIENT ENRICHMENT ; MASS-SPECTROMETRY ; GLYCOPROTEINS ; EXTRACTION ; QUANTIFICATION ; IDENTIFICATION ; RECOGNITION ; ADSORBENT ; SAMPLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000406764200016
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149794]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China 2.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China 3.Beijing Inst Radiat Med, State Key Lab Prote, Natl Ctr Prot Sci Beijing, Beijing 102200, Peoples R China
推荐引用方式 GB/T 7714	Qian, Xiaohong,Jiao, Fenglong,Gao, Fangyuan,et al. Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides[J]. SCIENTIFIC REPORTS,2017,7.
APA	Qian, Xiaohong.,Jiao, Fenglong.,Gao, Fangyuan.,Wang, Heping.,Deng, Yulin.,...&Zhang, Yukui.(2017).Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides.SCIENTIFIC REPORTS,7.
MLA	Qian, Xiaohong,et al."Polymeric hydrophilic ionic liquids used to modify magnetic nanoparticles for the highly selective enrichment of N-linked glycopeptides".SCIENTIFIC REPORTS 7(2017).