Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling

	Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling
	Deng Yu-Lin 1; Shan Yi-Chu 2; Zheng Nai-Ren 1; Zhang Yu-Kui 1,2
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2017-10-01
卷号	45 期号:10 页码:1441-1447
关键词	Proteomics Isobaric labeling Identification of proteins
英文摘要	Isobaric peptide termini labeling (IPTL) is a technology which uses light and heavy isotopes to label C-terminus and N-terminus of peptides. As the masses of labeled peptides are equivalent, the complexity of sample is low when analyzing MS data produced by this technology. Besides, paired b and y ions are helpful while analyzing MS/MS data in this kind of experiments. On the basis of this, a novel scoring algorithm, all ions scoring algorithm (AISA), has been designed for IPTL experiments. The information of quantification and qualification can be acquired at the same time using AISA. On Q-Exactive HeLa 2D RPLC dataset, peptide spectrum matches (PSMs), distinct peptides and protein groups identified by AISA are 15%, 26% and 22% higher than Morpheus. On human-HCC-HL dataset, PSMs, distinct peptides and protein groups identified by AISA are 24%, 39% and 27% higher than Morpheus. Quantification ratio on Q-Exactive HeLa and human. HCC-HL datasets are 1.18 and 0.90, respectively, which are very close to 1. Besides, quantification ratios between 0.5 and 2.0 are 91% and 94%, respectively.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	TANDEM MASS TAGS ; QUANTITATIVE PROTEOMICS ; MS/MS ; SPECTROMETRY ; EXPRESSION ; MIXTURES
收录类别	SCI
语种	英语
WOS记录号	WOS:000414957900004
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149735]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Deng Yu-Lin,Shan Yi-Chu,Zheng Nai-Ren,et al. Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(10):1441-1447.
APA	Deng Yu-Lin,Shan Yi-Chu,Zheng Nai-Ren,&Zhang Yu-Kui.(2017).Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(10),1441-1447.
MLA	Deng Yu-Lin,et al."Novel Algorithm for Identification and Quantification of Proteins Based on Strategy of Isobaric Peptide Termini Labelling".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.10(2017):1441-1447.