Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird

	Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird
	Pan, Shengkai 1,2,13; Zhang, Tongzuo 12; Rong, Zhengqin 11; Hu, Li 2,10,13; Zhan, Xiangjiang 1,2; Bruford, Michael W.1,3; Dixon, Andrew 1,4,5; Deutschova, Lucia 6; Li, Xinhai 2; Lin, Zhenzhen 2
刊名	MOLECULAR ECOLOGY
	2017-06-01
卷号	26 期号:11 页码:2993-3010
关键词	adaptation colonization population transcriptome Qinghai-Tibetan Plateau RNA sequencing saker falcon
英文摘要	Low oxygen and temperature pose key physiological challenges for endotherms living on the Qinghai-Tibetan Plateau (QTP). Molecular adaptations to high-altitude living have been detected in the genomes of Tibetans, their domesticated animals and a few wild species, but the contribution of transcriptional variation to altitudinal adaptation remains to be determined. Here we studied a top QTP predator, the saker falcon, and analysed how the transcriptome has become modified to cope with the stresses of hypoxia and hypothermia. Using a hierarchical design to study saker populations inhabiting grassland, steppe/desert and highland across Eurasia, we found that the QTP population is already distinct despite having colonized the Plateau <2000years ago. Selection signals are limited at the cDNA level, but of only seventeen genes identified, three function in hypoxia and four in immune response. Our results show a significant role for RNA transcription: 50% of upregulated transcription factors were related to hypoxia responses, differentiated modules were significantly enriched for oxygen transport, and importantly, divergent EPAS1 functional variants with a refined co-expression network were identified. Conservative gene expression and relaxed immune gene variation may further reflect adaptation to hypothermia. Our results exemplify synergistic responses between DNA polymorphism and RNA expression diversity in coping with common stresses, underpinning the successful rapid colonization of a top predator onto the QTP. Importantly, molecular mechanisms underpinning highland adaptation involve relatively few genes, but are nonetheless more complex than previously thought and involve fine-tuned transcriptional responses and genomic adaptation.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
关键词[WOS]	PRINCIPAL COMPONENT ANALYSIS ; INDUCIBLE FACTOR-I ; HIGH-ALTITUDE ; DIFFERENTIAL EXPRESSION ; GENOME SCANS ; IMMUNE-RESPONSE ; R PACKAGE ; SEQ DATA ; HYPOXIA ; ADAPTATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000402040000013
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/7094]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Cardiff Univ, Inst Zool, Joint Lab Biocomplex Res, Beichen West Rd, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Zool, Key Lab Anim Ecol & Conservat Biol, Beichen West Rd, Beijing 100101, Peoples R China 3.Cardiff Univ, Cardiff Sch Biosci, Organisms & Environm Div, Cardiff CF10 3AX, S Glam, Wales 4.Environm Agcy Abu Dhabi, POB 45553,Al Mamoura Bldg A,Muroor Rd, Abu Dhabi, U Arab Emirates 5.Int Wildlife Consultants Ltd, POB 19, Carmarthen SA33 5YL, Dyfed, Wales 6.Raptor Protect Slovakia, Kuklovska 5, SK-84104 Bratislava 4, Slovakia 7.Beijing Normal Univ, Coll Life Sci, MOE Key Lab Biodivers Sci & Ecol Engn, Beijing 100875, Peoples R China 8.Beijing Normal Univ, Coll Life Sci, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China 9.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Minist Educ, Key Lab Biomed Informat Engn, Xian 710049, Peoples R China 10.BGI Shenzhen, Shenzhen 518083, Peoples R China
推荐引用方式 GB/T 7714	Pan, Shengkai,Zhang, Tongzuo,Rong, Zhengqin,et al. Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird[J]. MOLECULAR ECOLOGY,2017,26(11):2993-3010.
APA	Pan, Shengkai.,Zhang, Tongzuo.,Rong, Zhengqin.,Hu, Li.,Zhan, Xiangjiang.,...&Wu, Qi.(2017).Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird.MOLECULAR ECOLOGY,26(11),2993-3010.
MLA	Pan, Shengkai,et al."Population transcriptomes reveal synergistic responses of DNA polymorphism and RNA expression to extreme environments on the Qinghai-Tibetan Plateau in a predatory bird".MOLECULAR ECOLOGY 26.11(2017):2993-3010.