The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers

CORC > 上海光学精密机械研究所 > 中国科学院上海光学精密机械研究所 > 强场激光物理国家重点实验室

	The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers
	Wang, Xiongwei; Liu, Jun; Huang, Zheng; Wang, Shimiao; Wang, Jing
刊名	Laser Phys. Lett.
	2016
卷号	13 期号:9
通讯作者	jliu@siom.ac.cn
英文摘要	Chlorin e6 (Ce6), a large heterocyclic aromatic molecule, is a promising photosensitizer for photodynamic therapy (PDT). We propose an efficient nano-photosensitizer delivery system based on covalent interactions between Ce6 and polyethylenimine (PEI) coated carbon nanodots (CDots). We observed >50% Ce6 drug loading content for PEI, due to this compound's unique 'proton sponge effect.' We found that the covalently incorporated Ce6 molecules retained their functional properties for near-infrared (NIR) fluorescence imaging and PDT. The chemical characteristics of CDot-PEI-Ce6 and Ce6 were evaluated using different analytical methods, including transmission electron microscopy and UV-Visible absorption spectra. Time-correlated single photon counting (TCSPC) and fluorescence spectra were used to demonstrate that Ce6 successfully conjugated to the CDots. The high cellular uptake of CDots-PEI-Ce6 was confirmed using flow cytometry and confocal laser scanning microscopy. According to the MTT assay, the CDots-PEI-Ce6 exhibited low dark toxicity and efficient PDT efficacy to HeLa cancer cells. These results indicate that CDot-PEI-Ce6 conjugates are potential photosensitizer delivery systems for PDT.
收录类别	SCI
资助信息	National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538]
WOS记录号	WOS:000383980900011
内容类型	期刊论文
源URL	[http://ir.siom.ac.cn/handle/181231/27743]
专题	上海光学精密机械研究所_强场激光物理国家重点实验室
作者单位	中国科学院上海光学精密机械研究所
推荐引用方式 GB/T 7714	Wang, Xiongwei,Liu, Jun,Huang, Zheng,et al. The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers[J]. Laser Phys. Lett.,2016,13(9).
APA	Wang, Xiongwei,Liu, Jun,Huang, Zheng,Wang, Shimiao,&Wang, Jing.(2016).The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers.Laser Phys. Lett.,13(9).
MLA	Wang, Xiongwei,et al."The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers".Laser Phys. Lett. 13.9(2016).