Fluid shear stress inhibits TNF-alpha-induced osteoblast apoptosis via ERK5 signaling pathway

doi:10.1016/j.bbrc.2015.08.117

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	Fluid shear stress inhibits TNF-alpha-induced osteoblast apoptosis via ERK5 signaling pathway
	Geng, B; Wang, CF; Zhang, B; Wang, J; Jiang, J; Tan, XY; Chen, C; Chen, YG; An, LP; Ma, JL
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2015-10-09
卷号	466 期号:1 页码:117-123
关键词	Fluid shear stress Osteoblast Extracellular signal-regulated kinase 5 Apoptosis TNF-alpha
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2015.08.117
文献子类	Article
英文摘要	Fluid shear stress (FSS) is a potent mechanical stimulus and prevents cells from TNF-alpha-induced apoptosis. Recently, Extracellular-signal-regulated kinase 5 (ERK5) has been found to be involved in regulation of cell survival. However, little is known about the role of ERK5 signaling pathway in FSS-mediated antiapoptotic effects in osteoblast In this study, we show that FSS blocks TNF-alpha-induced apoptosis of MC3T3-E1 cells via ERK5 signaling pathway. We found that physiological FSS for 1 h significantly decreased TNF-alpha-induced MC3T3-E1 cells apoptosis. After inhibition of ERK5 activity by XMD8-92, a highly-selective inhibitor of ERK5 activity, the ability of FSS to inhibit TNF-alpha induced apoptosis was significantly decreased. Analysis of anti-apoptotic mechanisms indicated that exposure of MC3T3-E1 cells to FSS for 1 h increased phosphorylation of Bad and inhibited caspase-3 activity. After treatment with XMD8-92, phosphorylation of Bad by FSS was significantly blocked, but caspase-3 activity was increased. In summary, these findings indicated that FSS inhibits INF-cc-mediated signaling events in osteoblast by a mechanism dependent on activation of ERK5, and Bad is a crucial downstream target for ERK5. Those results implied that ERK5 signaling pathway play a crucial role in FSS-mediated anti-apoptotic effect in osteoblast. Thus, ERK5 signaling pathway may be a new drug treatment target of osteoporosis and related bone-wasting diseases. (C) 2015 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
出版地	SAN DIEGO
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [81450042, 81071478]
语种	英语
WOS记录号	WOS:000362155000018
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179402]
专题	第二临床医学院_期刊论文
通讯作者	Xia, YY (reprint author), Lanzhou Univ, Hosp 2, Dept Orthopaed, 82 Cuiyingmen, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Geng, B,Wang, CF,Zhang, B,et al. Fluid shear stress inhibits TNF-alpha-induced osteoblast apoptosis via ERK5 signaling pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,466(1):117-123.
APA	Geng, B.,Wang, CF.,Zhang, B.,Wang, J.,Jiang, J.,...&Xia, YY .(2015).Fluid shear stress inhibits TNF-alpha-induced osteoblast apoptosis via ERK5 signaling pathway.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,466(1),117-123.
MLA	Geng, B,et al."Fluid shear stress inhibits TNF-alpha-induced osteoblast apoptosis via ERK5 signaling pathway".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 466.1(2015):117-123.