Interleukin-28B Plays a Therapeutic Role on Mouse U14 Cervical Cancer Cells by Down-Regulating CD4(+)CD25(+)FoxP3(+)Regulatory T Cells In Vivo

doi:10.1097/IGC.0000000000000528

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	Interleukin-28B Plays a Therapeutic Role on Mouse U14 Cervical Cancer Cells by Down-Regulating CD4(+)CD25(+)FoxP3(+)Regulatory T Cells In Vivo
	Chen, XY; Zhu, BD; Luo, YP; Zhang, DD; Zhang, L; Zhu, HH; Hao, CY; Guo, YZ; Liu, HL; Guo, YZ (reprint author), Lanzhou Univ, Affiliated Hosp 2, Dept Gynecol & Obstet, Lanzhou 730000, Gansu, Peoples R China.
刊名	INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
	2015-10
卷号	25 期号:8 页码:1369-1376
关键词	IL-28B Cervical cancer Regulatory T cells Immunotherapy U14 cell
ISSN号	1048-891X
DOI	10.1097/IGC.0000000000000528
文献子类	Article
英文摘要	Aim To investigate the immunotherapeutic effectiveness of adenoviral vector expressing mouse interleukin (IL)-28B (Ad-mIL-28B) against cervical cancer and its mechanism. Method U14 cervical cancer cell-bearing mice were treated with Ad-mIL-28B. Meanwhile, whole cell vaccine was prepared by repeated freezing and thawing U14 cells. Then CD4(+)CD25(+)FoxP3(+)regulatory T (Treg) cells were evaluated by flow cytometry. Tumor volume and metastasis in BALB/c and C57BL/6j mice were detected. Results Ad-mIL-28B treatment significantly decreased the number of CD4(+)CD25(+)FoxP3(+)Treg cells. Subsequently, there was a significant decrease in the size of tumor tissue and the numbers of heteromorphic tumor cells. The tumor metastasis in the lung and liver of the Ad-mIL-28B group also decreased. However, there was no therapeutic effect observed for whole cell vaccine on U14 tumor-bearing mice. Conclusion Interleukin-28B can inhibit the growth and metastasis of cervical cancer in U14 tumor-bearing mice by down-regulating Treg cells.
学科主题	Oncology ; Obstetrics & Gynecology
出版地	PHILADELPHIA
资助项目	国家自然科学基金项目 ; 甘肃省技术研究与开发专项计划
项目编号	National Natural Science Foundation of China [81072499] ; Gansu Province Science and Technology Project of China [1105TCYA016]
语种	英语
WOS记录号	WOS:000362177800006
资助机构	NSFC ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179398]
专题	第二临床医学院_期刊论文
通讯作者	Guo, YZ (reprint author), Lanzhou Univ, Affiliated Hosp 2, Dept Gynecol & Obstet, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Chen, XY,Zhu, BD,Luo, YP,et al. Interleukin-28B Plays a Therapeutic Role on Mouse U14 Cervical Cancer Cells by Down-Regulating CD4(+)CD25(+)FoxP3(+)Regulatory T Cells In Vivo[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2015,25(8):1369-1376.
APA	Chen, XY.,Zhu, BD.,Luo, YP.,Zhang, DD.,Zhang, L.,...&Guo, YZ .(2015).Interleukin-28B Plays a Therapeutic Role on Mouse U14 Cervical Cancer Cells by Down-Regulating CD4(+)CD25(+)FoxP3(+)Regulatory T Cells In Vivo.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,25(8),1369-1376.
MLA	Chen, XY,et al."Interleukin-28B Plays a Therapeutic Role on Mouse U14 Cervical Cancer Cells by Down-Regulating CD4(+)CD25(+)FoxP3(+)Regulatory T Cells In Vivo".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 25.8(2015):1369-1376.