ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast

doi:10.1007/s11010-015-2441-z

CORC > 兰州大学 > 兰州大学 > 第二临床医学院 > 期刊论文

	ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast
	Jiang, J; Zhao, LG; Teng, YJ; Chen, SL; An, LP; Ma, JL; Wang, J; Xia, YY; Xia, YY (reprint author), Lanzhou Univ, Hosp 2, Dept Orthopaed, 82 Cuiyingmen, Lanzhou, Gansu, Peoples R China.
刊名	MOLECULAR AND CELLULAR BIOCHEMISTRY
	2015-08
卷号	406 期号:1-2 页码:237-243
关键词	Fluid shear stress Osteoblast Extracellular-signal-regulated kinase 5 (ERK5) Cyclooxygenase-2 (COX-2) Transcription factors
ISSN号	0300-8177
DOI	10.1007/s11010-015-2441-z
文献子类	Article
英文摘要	Bone cells respond to various mechanical stimuli including fluid shear stress (FSS) in vitro. Induction of cyclooxygenase-2 (COX-2) is thought to be important for the anabolic effects of mechanical loading. Recently, extracellular-signal-regulated kinase 5 (ERK5) has been found to be involved in multiple cellular processes. However, the relationship between ERK5 and the induction of COX-2 is still unknown. Here, we investigated the potential involvement of ERK5 in the response of pre-osteoblastic MC3T3-E1 cells upon FSS. MC3T3-E1 cells were subjected to 12 dyn/cm(2) FSS. Then, we established a ERK5 small interfering RNA (siRNA) transfected cell line using the MC3T3-E1 cells. After the successful transfection confirmed by real-time reverse transcription-polymerase chain reaction and Western blotting, the expression of COX-2, cAMP response element-binding protein (CREB), and nuclear factor kappa B cells (NF-kappa B) were assayed for downstream effectors of activated ERK5 under FSS by Western blotting. Our results showed that FSS could stimulate COX-2 activity, and induce the phosphorylation of ERK5, CREB, and NF-kappa B. When the MC3T3-E1 cells were transfected using siRNA before exposure to FSS, COX-2 activity was suppressed, and the phosphorylation of CREB and NF-kappa B was significantly downregulated. In summary, we demonstrated that ERK5 pathway is essential in the induction of COX-2 gene.
学科主题	Cell Biology
出版地	DORDRECHT
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [81071478, 81450042]
语种	英语
WOS记录号	WOS:000358211800024
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179386]
专题	第二临床医学院_期刊论文
通讯作者	Xia, YY (reprint author), Lanzhou Univ, Hosp 2, Dept Orthopaed, 82 Cuiyingmen, Lanzhou, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Jiang, J,Zhao, LG,Teng, YJ,et al. ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2015,406(1-2):237-243.
APA	Jiang, J.,Zhao, LG.,Teng, YJ.,Chen, SL.,An, LP.,...&Xia, YY .(2015).ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast.MOLECULAR AND CELLULAR BIOCHEMISTRY,406(1-2),237-243.
MLA	Jiang, J,et al."ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast".MOLECULAR AND CELLULAR BIOCHEMISTRY 406.1-2(2015):237-243.