A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues

doi:10.1159/000439268

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	A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues
	Yan, ZJ; Yin, LJ; Wang, ZL; Ye, JX; Zhang, ZL; Li, RF; Denduluri, SK; Wang, J; Wei, Q; Zhao, LG
刊名	CELLS TISSUES ORGANS
	2015
卷号	201 期号:1 页码:38-50
关键词	Intervertebral disc Nucleus pulposus Annulus fibrosus Organ culture Recombinant adenovirus Tissue engineering Gene delivery
ISSN号	1422-6405
DOI	10.1159/000439268
文献子类	Article
英文摘要	The intervertebral disc (IVD) is a fibrocartilaginous joint between two vertebral bodies. An IVD unit consists of a gelatinous central nucleus pulposus, encased by the annulus fibrosus, which is sandwiched between cartilaginous endplates (EPs). The IVD homeostasis can be disrupted by injuries, ageing and/or genetic predispositions, leading to degenerative disc disorders and subsequent lower back pain. The complex structure and distinct characteristics of IVDs warrant the establishment of robust in vitro IVD organ culture for studying the etiology and treatment of disc degeneration. Here, we isolate mouse lumbar IVDs and culture the minimal IVD units in submersion or suspension medium supplemented with 2% bovine serum or 10% fetal bovine serum (FBS). We find the minimal IVD units remain healthy for up to 14 days when cultured in submersion culture supplemented with 10% FBS. New bone formation in the EPs of the cultured IVDs can be assessed with calcein labeling. Furthermore, the cultured IVDs can be effectively transduced by recombinant adenovirus, and transgene expression lasts for 2 weeks. Thus, our findings demonstrate that the optimized IVD organ culture system can be used to study IVD biology and screen for biological factors that may prevent, alleviate and/or treat disc degeneration. (C) 2015 S. Karger AG, Basel
学科主题	Anatomy & Morphology ; Cell Biology ; Developmental Biology
出版地	BASEL
资助项目	国家自然科学基金项目 ; 国家重点基础研究发展计划以及国家重大科学研究计划（973计划） ; 美国国立卫生研究院项目
项目编号	National Institutes of Health [AT004418] ; North American Spine Society ; Scoliosis Research Society ; 973 Program of Ministry of Science and Technology (MOST) of China [2011CB707900] ; Natural Science Foundation of China [81272005] ; University of Chicago Core Facility Subsidy grant from the National Center for Advancing Translational Sciences (NCATS) of the National Institutes of Health [UL1 TR000430]
语种	英语
WOS记录号	WOS:000368973100004
资助机构	NSFC ; MOST ; NIH
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179300]
专题	第二临床医学院_期刊论文
通讯作者	He, TC (reprint author), Univ Chicago, Med Ctr, Dept Orthopaed Surg & Rehabil Med, Mol Oncol Lab, 5841 South Maryland Ave,MC 3079, Chicago, IL 60637 USA.
推荐引用方式 GB/T 7714	Yan, ZJ,Yin, LJ,Wang, ZL,et al. A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues[J]. CELLS TISSUES ORGANS,2015,201(1):38-50.
APA	Yan, ZJ.,Yin, LJ.,Wang, ZL.,Ye, JX.,Zhang, ZL.,...&He, TC .(2015).A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues.CELLS TISSUES ORGANS,201(1),38-50.
MLA	Yan, ZJ,et al."A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues".CELLS TISSUES ORGANS 201.1(2015):38-50.