Alternol induces an S-phase arrest of melanoma B16F0 cells

doi:10.1002/cbin.10226

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	Alternol induces an S-phase arrest of melanoma B16F0 cells
	Liu, LL; Zhang, B; Yuan, X; Wang, PL; Sun, XL; Zheng, QS; Sun, XL (reprint author), Heart Spleen Fdn Tradit Chinese Med, Binzhou Med Colledge, Shandong Prov Key Lab, Yantai 264005, Peoples R China.
刊名	CELL BIOLOGY INTERNATIONAL
	2014-03
卷号	38 期号:3 页码:374-380
关键词	alternol B16F0 cell cycle S arrest
ISSN号	1065-6995
DOI	10.1002/cbin.10226
文献子类	Article
英文摘要	Alternol is a novel compound purified from the fermentation products of a microorganism in the yew tree bark. This study looks at the effects of alternol on the proliferation and cell cycle distribution of mouse melanoma cells. The inhibition of cell proliferation and changes in cell cycle distribution were analysed by sulforhodamine B and flow cytometry assays, respectively. mRNA expression of cyclin A, cyclin-dependent kinase 2 (CDK2), proliferating cell nuclear antigen (PCNA) and CDK inhibitor1A (p21) were measured by real-time reverse transcription PCR (RT-PCR). The protein levels of cyclin A, CDK2 and PCNA were analysed by Western blot analysis. p21 was measured by ELISA. Alternol treatment caused a significant decrease in the proliferation rate of B16F0 and B16F10 cells, which were significantly arrested in S phase, but this treatment had less effect on normal human embryonic kidney 293T cells. The mechanism by which alternol inhibits B16F0 proliferation in vitro may be associated with the inhibition of CDK2 and PCNA, and the activation of p21.
学科主题	Cell Biology
出版地	HOBOKEN
资助项目	国家科技重大专项
项目编号	Development of Major New Drugs of China [2009ZX09103] ; Outstanding Youth Research Program of Xinjiang Production and Construction Corps [2011CD006] ; International Scientific and Technological Cooperation Projects [2012BC001]
语种	英语
WOS记录号	WOS:000330558900011
资助机构	MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/126185]
专题	第二临床医学院_期刊论文
通讯作者	Sun, XL (reprint author), Heart Spleen Fdn Tradit Chinese Med, Binzhou Med Colledge, Shandong Prov Key Lab, Yantai 264005, Peoples R China.
推荐引用方式 GB/T 7714	Liu, LL,Zhang, B,Yuan, X,et al. Alternol induces an S-phase arrest of melanoma B16F0 cells[J]. CELL BIOLOGY INTERNATIONAL,2014,38(3):374-380.
APA	Liu, LL.,Zhang, B.,Yuan, X.,Wang, PL.,Sun, XL.,...&Sun, XL .(2014).Alternol induces an S-phase arrest of melanoma B16F0 cells.CELL BIOLOGY INTERNATIONAL,38(3),374-380.
MLA	Liu, LL,et al."Alternol induces an S-phase arrest of melanoma B16F0 cells".CELL BIOLOGY INTERNATIONAL 38.3(2014):374-380.