5-Azacytidine suppresses the proliferation of pancreatic cancer cells by inhibiting the Wnt/beta-catenin signaling pathway

doi:10.4238/2014.July.4.22

CORC > 兰州大学 > 兰州大学 > 第一临床医学院 > 期刊论文

	5-Azacytidine suppresses the proliferation of pancreatic cancer cells by inhibiting the Wnt/beta-catenin signaling pathway
	Zhang, H; Zhou, WC; Li, X; Meng, WB; Zhang, L; Zhu, XL; Zhu, KX; Bai, ZT; Yan, J; Liu, T
刊名	GENETICS AND MOLECULAR RESEARCH
	2014
卷号	13 期号:3 页码:5064-5072
关键词	5-Azacytidine c-Myc CyclinD1 Pancreatic cancer Wnt/beta-catenin
ISSN号	1676-5680
DOI	10.4238/2014.July.4.22
文献子类	Article
英文摘要	5-Azacytidine has been shown to be an effective anti-pancreatic cancer drug, but the mechanism remains unknown. In the current study, we explored the effect of 5-azacytidine on abnormal activation of the Wnt-beta-catenin signaling pathway in pancreatic cancer cells. The human pancreatic cancer cell line Bxpc-3 was treated with different concentrations of 5-azacytidine for various times. The proliferation and early apoptosis of the cells were evaluated using the CCK8 method and flow cytometry, respectively. mRNA and protein expression of beta-catenin, c-myc, and cyclinD1 were detected using real-time fluorescent quantitative polymerase chain reaction and Western blot analysis, respectively. The proliferation of Bxpc-3 cells was suppressed by 5-azacytidine. The early apoptosis of the cells was significantly enhanced over time and with increasing drug concentrations. The expression of beta-catenin, c-myc, and cyclinD1 were down-regulated, showing significant differences between different concentrations and treatment times (P < 0.05). 5-Azacytidine suppressed the proliferation of pancreatic cancer cells by inhibiting the Wnt/beta-catenin signaling pathway, particularly the expression of beta-catenin, c-myc, and cyclinD1. This study may provide a new potential strategy for diagnosing and treating pancreatic cancer.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
出版地	RIBEIRAO PRETO
资助项目	中央高校基本科研业务费专项资金 ; 甘肃省自然科学基金计划
项目编号	Fundamental Research Funds for the Central Universities [lzujbky-2012-163] ; West Light Foundation of the Chinese Academy of Science [2011-180] ; Gansu Provincial Natural Science Foundation [1208RJZA296]
语种	英语
WOS记录号	WOS:000340383600038
资助机构	LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125865]
专题	第一临床医学院_期刊论文
通讯作者	Li, YM (reprint author), Lanzhou Univ, Hosp 2, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Zhang, H,Zhou, WC,Li, X,et al. 5-Azacytidine suppresses the proliferation of pancreatic cancer cells by inhibiting the Wnt/beta-catenin signaling pathway[J]. GENETICS AND MOLECULAR RESEARCH,2014,13(3):5064-5072.
APA	Zhang, H.,Zhou, WC.,Li, X.,Meng, WB.,Zhang, L.,...&Li, YM .(2014).5-Azacytidine suppresses the proliferation of pancreatic cancer cells by inhibiting the Wnt/beta-catenin signaling pathway.GENETICS AND MOLECULAR RESEARCH,13(3),5064-5072.
MLA	Zhang, H,et al."5-Azacytidine suppresses the proliferation of pancreatic cancer cells by inhibiting the Wnt/beta-catenin signaling pathway".GENETICS AND MOLECULAR RESEARCH 13.3(2014):5064-5072.