Development and validation of a reversed phase liquid chromatographic method with fluorescence detection for the pharmacokinetic study of a new chimeric peptide

doi:10.1039/c5ay02770e

CORC > 兰州大学 > 兰州大学 > 药学院 > 期刊论文

	Development and validation of a reversed phase liquid chromatographic method with fluorescence detection for the pharmacokinetic study of a new chimeric peptide
	Zhang, XY; Chen, Y; Li, L; Qiao, H; Wang, R; Ni, JM; You, JQ; Ma, KK; Fan, CC; Fang, Q
刊名	ANALYTICAL METHODS
	2016
卷号	8 期号:12 页码:2620-2627
ISSN号	1759-9660
DOI	10.1039/c5ay02770e
文献子类	Article
英文摘要	The objective of this research is to develop a sensitive bioanalytical method and investigate the pharmacokinetics of a new chimeric peptide (BN-9) in rats. According to the analysis criteria, a reversed phase high performance liquid chromatography method with fluorescence detection (HPLC-FLD) was established and employed in monitoring the compound in rat plasma using doxorubicin (DOX) as the internal standard (IS). Fluorescent BN-9 and IS were extracted from plasma using dehydrated alcohol. The mobile phase was composed of acetonitrile and potassium dihydrogen phosphate buffer containing 0.05% trifluoroacetic acid (TFA) (pH 7.4; 0.02 M) (30 : 70, v/v) at 0.7 mL min(-1). Fluorescence detection was conducted at 490 nm (excitation wavelength) and 520 nm (emission wavelength). The lower limit of quantification (LLOQ) was 0.009 mu g mL(-1). The intra-batch and inter-batch precisions were less than 8.116%, and the accuracy was within 5.632%. Blood samples were collected after intravenous administration of fluorescent BN-9 at the doses of 5, 10, and 20 mg kg(-1) to rats. The main pharmacokinetic parameters were obtained by non-compartmental and compartmental analysis with DAS 2.1.1 software. The t(1/2 alpha) values were 9.584 +/- 5.137, 8.548 +/- 2.093 and 9.621 +/- 0.3224 min. The results showed that the method was successfully applied to investigate the pharmacokinetic profiles of fluorescent BN-9 following intravenous administration to rats.
学科主题	Chemistry ; Food Science & Technology ; Spectroscopy
出版地	CAMBRIDGE
资助项目	国家自然科学基金项目 ; 甘肃省科技支撑计划
项目编号	National Undergraduate Innovative and Entrepreneurial Training Program of Lanzhou University [201510730140] ; National Natural Science Foundation of China [81273440] ; Technology Support Projects of Gansu Province [1304FKCA073]
语种	英语
WOS记录号	WOS:000372758000009
资助机构	NSFC ; LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179530]
专题	药学院_期刊论文
通讯作者	Ni, JM (reprint author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Zhang, XY,Chen, Y,Li, L,et al. Development and validation of a reversed phase liquid chromatographic method with fluorescence detection for the pharmacokinetic study of a new chimeric peptide[J]. ANALYTICAL METHODS,2016,8(12):2620-2627.
APA	Zhang, XY.,Chen, Y.,Li, L.,Qiao, H.,Wang, R.,...&Ni, JM .(2016).Development and validation of a reversed phase liquid chromatographic method with fluorescence detection for the pharmacokinetic study of a new chimeric peptide.ANALYTICAL METHODS,8(12),2620-2627.
MLA	Zhang, XY,et al."Development and validation of a reversed phase liquid chromatographic method with fluorescence detection for the pharmacokinetic study of a new chimeric peptide".ANALYTICAL METHODS 8.12(2016):2620-2627.