Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes

doi:10.1016/j.actaastro.2016.09.017

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	Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes
	Chen, Zhihao; Zhao, Fan; Qi, Yiduo; Hu, Lifang; Li, Dijie; Yin, Chong; Su, Peihong; Zhang, Yan; Ma, Jianhua; Qian, Jing
刊名	Acta Astronautica
	2016-12-01
卷号	129 页码:186-192
关键词	Simulated microgravity Random position machine (RPM) Gene expression profile Osteocyte ATP-binding Cytoskeleton
ISSN号	0094-5765
DOI	10.1016/j.actaastro.2016.09.017
文献子类	Article
英文摘要	Bone undergoes dynamic modelling and remodelling processes, and it requires gravity-mediated mechanical stimulation for the maintenance of mineral content and structure. Osteocytes are the most commonly found cells in the mature bone, and they are sensitive to mechanical changes. The purpose of this study was to investigate the effects of microgravity simulated with a random position machine (RPM) on the gene expression profile of osteocytes. Genes sensitive to RPM treatment were sorted on the basis of biological processes, interactions and signalling pathways. Overall, 504 differentially expressed genes (DEGs) in osteocytes cultured under RPM conditions were found. The DEGs were further analysed using bioinformatics tools such as DAVID and iReport. A total of 15 ATP-binding and cytoskeleton-related genes were further confirmed by quantitative real-time PCR (qRT-PCR). Our findings demonstrate that the RPM affected the expression of genes involved in cytoskeleton remodelling and the energy-transfer process in osteocytes. The identification of mechanosensitive genes may enhance our understanding of the roles of osteocytes in mechanosensation and may provide some potential targets for preventing and treating bone-related diseases.
学科主题	Engineering
出版地	OXFORD
资助项目	国家自然科学基金项目 ; 新世纪优秀人才支持计划 ; 中央高校基本科研业务费专项资金
项目编号	Natural Science Foundation of China [31570940, 31400725] ; Natural Science Basic Research Plan in Shaanxi Province of China [2015JM3078, 2015JQ3076] ; Fundamental Research Funds for the Central Universities [lzujbky-2015-188] ; New Century Excellent Talents in University [NCET-12-0469]
语种	英语
WOS记录号	WOS:000389087900021
资助机构	NSFC ; MOE ; LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179637]
专题	公共卫生学院_期刊论文
通讯作者	Qian, AR (reprint author), Northwestern Polytech Univ, Sch Life Sci, Inst Special Environm Biophys, Key Lab Space Biosci & Biotechnol, Xian 710072, Peoples R China.
推荐引用方式 GB/T 7714	Chen, Zhihao,Zhao, Fan,Qi, Yiduo,et al. Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes[J]. Acta Astronautica,2016,129:186-192.
APA	Chen, Zhihao.,Zhao, Fan.,Qi, Yiduo.,Hu, Lifang.,Li, Dijie.,...&Qian, AR .(2016).Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes.Acta Astronautica,129,186-192.
MLA	Chen, Zhihao,et al."Simulated microgravity alters the expression of cytoskeleton- and ATP-binding-related genes in MLO-Y4 osteocytes".Acta Astronautica 129(2016):186-192.