Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application

doi:10.1080/10826076.2015.1020164

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	Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application
	Liu, YW; Bu, LN; Zhao, JX; Wei, JT; Liu, YW (reprint author), Lanzhou Univ, Sch Publ Hlth, Inst Nutr & Food, Hyg, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China.
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2015-07-21
卷号	38 期号:12 页码:1179-1184
关键词	metabolomics antioxidant capacity intracellular metabolite evaluation approach biomarker
ISSN号	1082-6076
DOI	10.1080/10826076.2015.1020164
文献子类	Article
英文摘要	Metabolomics is frequently used in the analysis of the dynamic changes within small molecules in response to the disturbance in an organism. It has been extensively used in various fields involving human diseases. To better evaluate the antioxidant capacity, cells subjected to oxidative stress were cultured, and the intracellular metabolites were prepared using HSDE (high-speed shear dispersing emulsifier)-assisted extraction. The metabolites were then analyzed under optimized chromatographic conditions. Partial least squares discriminate analysis was used to analyze the data. A new intracellular metabolomic approach was established, and four potential metabolic biomarkers for repairing oxidative stress were found. The antioxidant activities of Cynomorium songaricum was successfully evaluated using the intracellular metabolomic approach, indicating the feasibility of the proposed approach as an effective tool for evaluating the antioxidant capacity.
学科主题	Biochemistry & Molecular Biology ; Chemistry
出版地	PHILADELPHIA
资助项目	国家自然科学基金项目 ; 国家高技术研究发展计划（863计划） ; 甘肃省青年科技基金计划
项目编号	National Natural Sciences Foundation of China [20974116, 21175142] ; National High Technology Research and Development Program of China [2014AA022203] ; Science Foundation of Gansu Province [1308RJYA077]
语种	英语
WOS记录号	WOS:000355595800002
资助机构	NSFC ; MOST ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179611]
专题	公共卫生学院_期刊论文
通讯作者	Liu, YW (reprint author), Lanzhou Univ, Sch Publ Hlth, Inst Nutr & Food, Hyg, 222 Tianshui South Rd, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Liu, YW,Bu, LN,Zhao, JX,et al. Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015,38(12):1179-1184.
APA	Liu, YW,Bu, LN,Zhao, JX,Wei, JT,&Liu, YW .(2015).Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,38(12),1179-1184.
MLA	Liu, YW,et al."Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 38.12(2015):1179-1184.