4-isothiocyanate-2, 2, 6, 6-tetramethyl piperidinooxyl inhibits angiogenesis by suppressing VEGFR2 and Tie2 phosphorylation

doi:10.3892/ol.2016.4948

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	4-isothiocyanate-2, 2, 6, 6-tetramethyl piperidinooxyl inhibits angiogenesis by suppressing VEGFR2 and Tie2 phosphorylation
	Liu, YY; Gao, J; Huang, SS; Hu, LM; Wang, ZQ; Wang, ZY; Chen, X; Zhang, XY; Li, WG; Li, WG (reprint author), Lanzhou Univ, Coll Basic Med, Key Lab Preclin Study New Drugs Gansu, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.
刊名	ONCOLOGY LETTERS
	2016-10
卷号	12 期号:4 页码:2828-2834
关键词	4-isothiocyanate-2 2 6 6-tetramethyl piperidinooxyl angiogenesis reactive oxygen species vascular endothelial growth factor receptor 2 Tie2
ISSN号	1792-1074
DOI	10.3892/ol.2016.4948
文献子类	Article
英文摘要	Reactive oxygen species (ROS) are involved in the signaling pathway and are triggered by angiogenic factors, including vascular endothelial growth factor and angiopoietins. 4-isothiocyanate-2, 2, 6, 6-tetramethyl piperidinooxyl (4-ISO-Tempo) is one of the nitroxides that exhibits antioxidant activity. However, the anti-angiogenic effect of 4-ISO-Tempo remains unknown. The aim of this study was to investigate the effect of 4-ISO-Tempo on tumor proliferation and angiogenesis as well as its underlying mechanisms. Our results revealed that 4-ISO-Tempo significantly inhibited the viability of neoplastic and endothelial cells. Furthermore, the effective concentration of 4-ISO-Tempo on human microvascular endothelial cell 1 (HMEC-1) was lower than that on human lung adenocarcinoma A549 and human colon cancer SW620 cells. This suggested that endothelial cells were more sensitive to 4-ISO-Tempo than tumor cells. Furthermore, we demonstrated that 4-ISO-Tempo also suppressed secretion of matrix metalloproteinase (MMP)-2 and MMP-9, and migration and tube formation of HMEC-1 cells. The mechanism is attributed to the decreasing ROS generation and further phosphorylation of vascular endothelial growth factor receptor 2 and Tie2. Our findings suggest that 4-ISO-Tempo should be investigated for its usefulness in anti-angiogenesis therapies.
学科主题	Oncology
出版地	ATHENS
资助项目	国家自然科学基金项目 ; 兰州大学医学科研基金 ; 甘肃省重点实验室资助项目
项目编号	Medical Foundation of Lanzhou University [LZUYX200617] ; Key Lab of Preclinical Study for New Drugs of Gansu Province [GSKFKT-0702] ; National Natural Science Foundation of China [30700142]
语种	英语
WOS记录号	WOS:000385579200091
资助机构	NSFC ; LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188748]
专题	基础医学院_期刊论文
通讯作者	Li, WG (reprint author), Lanzhou Univ, Coll Basic Med, Key Lab Preclin Study New Drugs Gansu, 199 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Liu, YY,Gao, J,Huang, SS,et al. 4-isothiocyanate-2, 2, 6, 6-tetramethyl piperidinooxyl inhibits angiogenesis by suppressing VEGFR2 and Tie2 phosphorylation[J]. ONCOLOGY LETTERS,2016,12(4):2828-2834.
APA	Liu, YY.,Gao, J.,Huang, SS.,Hu, LM.,Wang, ZQ.,...&Li, WG .(2016).4-isothiocyanate-2, 2, 6, 6-tetramethyl piperidinooxyl inhibits angiogenesis by suppressing VEGFR2 and Tie2 phosphorylation.ONCOLOGY LETTERS,12(4),2828-2834.
MLA	Liu, YY,et al."4-isothiocyanate-2, 2, 6, 6-tetramethyl piperidinooxyl inhibits angiogenesis by suppressing VEGFR2 and Tie2 phosphorylation".ONCOLOGY LETTERS 12.4(2016):2828-2834.