Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA

doi:10.3389/fmicb.2016.01625

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	Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
	Xu, T; Han, J; Zhang, J; Chen, J; Wu, N; Zhang, W; Zhang, Y; Zhang, Y (reprint author), Fudan Univ, Shanghai Med Coll, Huashan Hosp, Key Lab Med Mol Virol, Shanghai, Peoples R China.; Zhang, Y (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21218 USA.
刊名	FRONTIERS IN MICROBIOLOGY
	2016-10-24
卷号	7
关键词	Staphylococcus aureus heme antibiotics pigment virulence persister formation
ISSN号	1664-302X
DOI	10.3389/fmicb.2016.01625
文献子类	Article
英文摘要	The membrane protein CtaB in S. aureus is a protoheme IX farnesyltransferase involved in the synthesis of the heme containing terminal oxidases of bacterial respiratory chain. In this study, to assess the role of CtaB in S. aureus virulence, pigment production, and persister formation, we constructed a ctaB mutant in the methicillin-resistant Staphylococcus aureus (MRSA) strain USA500. We found that deletion of ctaB attenuated growth and virulence in mice but enhanced pigment production and formation of quinolone tolerant persister cells in stationary phase. RNA-seq analysis showed that deletion of ctaB caused decreased transcription of several virulence genes including RNAIII which is consistent with its virulence attenuation. In addition, transcription of 20 ribosomal genes and 24 genes involved in amino acid biosynthesis was significantly down-regulated in the ctaB knockout mutant compared with the parent strain. These findings suggest the importance of heme biosynthesis in virulence and persister formation of S. aureus.
学科主题	Microbiology
出版地	LAUSANNE
资助项目	国家自然科学基金项目 ; 国家科技重大专项 ; 美国国立卫生研究院项目
项目编号	National Natural Science Foundation of China [81572046, 81471987] ; Key Technologies Research and Development Program for Infectious Diseases of China [2013ZX10003008-003] ; NIH [AI99512, AI108535]
语种	英语
WOS记录号	WOS:000388631700001
资助机构	NSFC ; MOST ; NIH
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188736]
专题	基础医学院_期刊论文
通讯作者	Zhang, W; Zhang, Y (reprint author), Fudan Univ, Shanghai Med Coll, Huashan Hosp, Key Lab Med Mol Virol, Shanghai, Peoples R China.; Zhang, Y (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21218 USA.
推荐引用方式 GB/T 7714	Xu, T,Han, J,Zhang, J,et al. Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA	Xu, T.,Han, J.,Zhang, J.,Chen, J.,Wu, N.,...&Zhang, Y .(2016).Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA.FRONTIERS IN MICROBIOLOGY,7.
MLA	Xu, T,et al."Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA".FRONTIERS IN MICROBIOLOGY 7(2016).