Differential expression and response to arsenic stress of MRPs and ASAN1 determine sensitivity of classical multidrug-resistant leukemia cells to arsenic trioxide

doi:10.1016/j.leukres.2016.10.003

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	Differential expression and response to arsenic stress of MRPs and ASAN1 determine sensitivity of classical multidrug-resistant leukemia cells to arsenic trioxide
	Chen, J; Cheng, J; Yi, J; Xie, B; Lin, L; Liu, Z; Zhao, HS; Wang, B; Ai, ZY; Yang, Y
刊名	LEUKEMIA RESEARCH
	2016-11
卷号	50 页码:116-122
关键词	Arsenic-resistance Multi-drug resistance MRP1 MRP2 ASNA1 Leukemia cella
ISSN号	0145-2126
DOI	10.1016/j.leukres.2016.10.003
文献子类	Article
英文摘要	There is no cross-resistance between arsenic trioxide and conventional chemotherapeutics. Classical multi-drug resistant (MDR) cells remain sensitive to arsenic trioxide, which may even reverse the drug resistance. Arsenic trioxide is also effective in leukemias/tumors that persist despite conventional cytotoxic or targeted drugs. We obtained a highly arsenic-resistant MDR leukemic cell line, HL-60/RS, by exposing leukemic HL-60 cells to adriamycin selection. We compared the arsenic sensitivity, and the expression and responses to arsenic of the arsenic-related transporters, MRP1, MRP2, and ASNA1, in paired parent/arsenic-resistant HL-60/RS/HL-60 and arsenic-sensitive/parental K562/ADM/K562 cells. Expression levels of MRP1, MRP2, and ASNA1 were negatively correlated with cell sensitivities to arsenic trioxide, and ASNA1 expression notably was highest in HL-60/RS cells and lowest in K562/ADM cells. Expression levels of MRP1, MRP2, and ASNA1 were significantly enhanced in HL-60/RS cells and inhibited in K562/ADM cells by arsenic trioxide treatment, compared with their parental sensitive cells, in accord with the high-resistance of HL-60/RS cells and high-sensitivity of K562/ADM cells. In conclusion, the cross-resistance of conventional chemotherapeutics-resistant leukemic cells to arsenic trioxide is determined by both levels of MRP1, MRP2, and ASNA1, and also by the responses of these transporters to arsenic stress. (C) 2016 Elsevier Ltd. All rights reserved.
学科主题	Oncology ; Hematology
出版地	OXFORD
资助项目	国家自然科学基金项目 ; 中央高校基本科研业务费专项资金 ; 兰州市科技局资助项目
项目编号	National Natural Science Foundation of China [81541025] ; Fundamental Research Funds for Central Universities [lzujbky-2016-174] ; Science and Technology Planning Project from Chengguan District of Lanzhou City of Gansu Province in China
语种	英语
WOS记录号	WOS:000388315500018
资助机构	NSFC ; LZU ; LZSTB
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188734]
专题	基础医学院_期刊论文
通讯作者	Wei, HL (reprint author), Lanzhou Univ, 199 Donggang West Rd, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Chen, J,Cheng, J,Yi, J,et al. Differential expression and response to arsenic stress of MRPs and ASAN1 determine sensitivity of classical multidrug-resistant leukemia cells to arsenic trioxide[J]. LEUKEMIA RESEARCH,2016,50:116-122.
APA	Chen, J.,Cheng, J.,Yi, J.,Xie, B.,Lin, L.,...&Wei, HL .(2016).Differential expression and response to arsenic stress of MRPs and ASAN1 determine sensitivity of classical multidrug-resistant leukemia cells to arsenic trioxide.LEUKEMIA RESEARCH,50,116-122.
MLA	Chen, J,et al."Differential expression and response to arsenic stress of MRPs and ASAN1 determine sensitivity of classical multidrug-resistant leukemia cells to arsenic trioxide".LEUKEMIA RESEARCH 50(2016):116-122.