Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment

doi:10.3892/or.2016.4884

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	Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment
	Hu, KY; Wang, DG; Liu, PF; Cao, YW; Wang, YH; Yang, XC; Hu, CX; Sun, LJ; Niu, HT; Niu, HT (reprint author), Qingdao Univ, Affiliated Hosp, Dept Urol, 59 Haier Rd, Qingdao 266101, Shandong, Peoples R China.
刊名	ONCOLOGY REPORTS
	2016-08
卷号	36 期号:2 页码:945-951
关键词	bladder cancer tumor microenvironment PFKFB3 MCT1 proliferation apoptosis
ISSN号	1021-335X
DOI	10.3892/or.2016.4884
文献子类	Article
英文摘要	Phosphofructokinase-2/fructose-2,6-bisphosphatase 3 (PFKFB3) and monocarboxylate transporter 1 (MCT1) play important roles in tumor endothelial cells (ECs) and several biological processes. The present study was conducted to study the effects of PFKFB3 and MCT1 on cell proliferation and apoptosis in the tumor microenvironment by co-culture of HUVECs and T24, a bladder cancer (BC) cell line, using a microfluidic device. Immunofluorescence assay showed that HUVEC activity was significantly enhanced under co-culture with T24 cells, according to the stronger fluorescence intensity of CD31 and CD105 than that in the signal-cultured cells. Quercetin treatment inhibited MCT1 expression but did not affect PFKFB3 expression. Knockdown of MCT1 or/and PFKFB3 increased the apoptosis rate of the HUVECs under single-culture and co-culture situations by staining with calcein and propidium iodide. Meanwhile, cell proliferation and lactic concentration were significantly decreased after the blocking of MCT1 or/and PFKFB3, as compared with that in the control group. No obvious differences in the effects on apoptosis, proliferation and lactic concentration were found between cells treated with quercetin and siMCT1. Thus, we concluded that the targeting of MCT1 and PFKFB3 regulated tumor microenvironment, and quercetin exhibited a potential antitumor effect by targeting MCT1.
学科主题	Oncology
出版地	ATHENS
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [30901481, 81372752, 81472411] ; Wu Jieping Medical Foundation [320.6750.13261] ; Natural Science Foundation of Shandong Province, China [ZR2014HM088]
语种	英语
WOS记录号	WOS:000379248700041
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179135]
专题	基础医学院_期刊论文
通讯作者	Sun, LJ; Niu, HT (reprint author), Qingdao Univ, Affiliated Hosp, Dept Urol, 59 Haier Rd, Qingdao 266101, Shandong, Peoples R China.
推荐引用方式 GB/T 7714	Hu, KY,Wang, DG,Liu, PF,et al. Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment[J]. ONCOLOGY REPORTS,2016,36(2):945-951.
APA	Hu, KY.,Wang, DG.,Liu, PF.,Cao, YW.,Wang, YH.,...&Niu, HT .(2016).Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment.ONCOLOGY REPORTS,36(2),945-951.
MLA	Hu, KY,et al."Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment".ONCOLOGY REPORTS 36.2(2016):945-951.