SIMULTANEOUS DETERMINATION OF GUANINE, URIC-ACID, HYPOXANTHINE AND XANTHINE IN HUMAN PLASMA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION

	SIMULTANEOUS DETERMINATION OF GUANINE, URIC-ACID, HYPOXANTHINE AND XANTHINE IN HUMAN PLASMA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION
	LIU ZM ; LI T ; WANG E
刊名	analyst
	1995
卷号	120 期号:8 页码:2181-2184
关键词	QUANTITATION PURINE FLUIDS
ISSN号	0003-2654
中文摘要	a reversed-phase high-performance liquid chromatographic method with amperometric detection is described for the separation and quantification of uric acid, guanine, hypoxanthine and xanthine. the isocratic separation of a standard mixture of the compounds was achieved in 5 min on a spherisorb 5 c-18 reversed-phase column, with a mobile phase of nah2po4 (300 mmol dm(-3) ph 3.0)-methanol-acetonitrile-tetrahydrofuran (97.8 + 0.5 + 1.5 + 0.2). uric acid, guanine, hypoxanthine and xanthine were completely separated, with detection limits in the range 2-20 pmol per injection. the effect of ph and the composition of the mobile phase on the separation are described. the hydrodynamic voltammograms of these compounds were recorded at a glassy carbon electrode. the linear range of the calibration graph for each compound was: uric acid; 1-5000 mu mol dm(-3); guanine, 0.5-2000 mu mol dm(-3); hypoxanthine, 0.1-500 mu mol dm(-3) and xanthine, 0.5-5000 mu mol dm(-3). the within- and between-day precision was good. the uric acid and hypoxanthine content in human plasma was measured using the proposed method. good recoveries of uric acid (97.9-103%), hypoxanthine (98.0-99.2%), guanine (96.0-98.3%) and xanthine (96.0-102%) were obtained from human plasma. the results of electrochemical detection were in good agreement with those of uv detection.
收录类别	SCI收录期刊论文
语种	英语
公开日期	2010-12-30 ; 2011-06-10
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/26489]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	LIU ZM,LI T,WANG E. SIMULTANEOUS DETERMINATION OF GUANINE, URIC-ACID, HYPOXANTHINE AND XANTHINE IN HUMAN PLASMA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION[J]. analyst,1995,120(8):2181-2184.
APA	LIU ZM,LI T,&WANG E.(1995).SIMULTANEOUS DETERMINATION OF GUANINE, URIC-ACID, HYPOXANTHINE AND XANTHINE IN HUMAN PLASMA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION.analyst,120(8),2181-2184.
MLA	LIU ZM,et al."SIMULTANEOUS DETERMINATION OF GUANINE, URIC-ACID, HYPOXANTHINE AND XANTHINE IN HUMAN PLASMA BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION".analyst 120.8(1995):2181-2184.