Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1 alpha expression | |
Wang, T; Zhou, YT; Chen, XN; Zhu, AX; Chen, XN (reprint author), LanZhou Univ, Sch Basic Med Sci, Inst Pathophysiol, Lanzhou, Gansu, Peoples R China. | |
刊名 | BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH |
2014-09 | |
卷号 | 47期号:9页码:738-745 |
关键词 | Hypoxia-inducible factor-1 alpha Ischemic postconditioning Skeletal muscle ischemia |
ISSN号 | 0100-879X |
DOI | 10.1590/1414-431X20142910 |
文献子类 | Article |
英文摘要 | Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is one of the most potent angiogenic growth factors. It improves angiogenesis and tissue perfusion in ischemic skeletal muscle. In the present study, we tested the hypothesis that ischemic postconditioning is effective for salvaging ischemic skeletal muscle resulting from limb ischemia-reperfusion injury, and that the mechanism involves expression of HIF-1 alpha. Wistar rats were randomly divided into three groups (n=36 each): sham-operated (group S), hindlimb ischemia-reperfusion (group IR), and ischemic postconditioning (group IPO). Each group was divided into subgroups (n=6) according to reperfusion time: immediate (0 h, T-0), 1 h (T-1), 3 h (T-3), 6 h (T-6), 12 h (T-12), and 24 h (T-24). In the IPO group, three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were carried out before reperfusion. At all reperfusion times (T-0-T-24), serum creatine kinase (CK) and lactate dehydrogenase (LDH) activities, as well as interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-alpha) concentrations, were measured in rats after they were killed. Histological and immunohistochemical methods were used to assess the skeletal muscle damage and HIF-1 alpha expression in skeletal muscle ischemia. In groups IR and IPO, serum LDH and CK activities and TNF-alpha, IL-6, and IL-10 concentrations were all significantly increased compared to group S, and HIF-1 alpha expression was up-regulated (P<0.05 or P<0.01). In group IPO, serum LDH and CK activities and TNF-alpha and IL-6 concentrations were significantly decreased, IL-10 concentration was increased, HIF-1 alpha expression was down-regulated (P<0.05 or P<0.01), and the pathological changes were reduced compared to group IR. The present study suggests that ischemic postconditioning can reduce skeletal muscle damage caused by limb ischemia-reperfusion and that its mechanisms may be related to the involvement of HIF-1 alpha in the limb ischemia-reperfusion injury-triggered inflammatory response. |
学科主题 | Life Sciences & Biomedicine - Other Topics ; Research & Experimental Medicine |
出版地 | SAO PAULO |
语种 | 英语 |
WOS记录号 | WOS:000340836500002 |
内容类型 | 期刊论文 |
源URL | [http://ir.lzu.edu.cn/handle/262010/121717] |
专题 | 基础医学院_期刊论文 |
通讯作者 | Chen, XN (reprint author), LanZhou Univ, Sch Basic Med Sci, Inst Pathophysiol, Lanzhou, Gansu, Peoples R China. |
推荐引用方式 GB/T 7714 | Wang, T,Zhou, YT,Chen, XN,et al. Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1 alpha expression[J]. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH,2014,47(9):738-745. |
APA | Wang, T,Zhou, YT,Chen, XN,Zhu, AX,&Chen, XN .(2014).Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1 alpha expression.BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH,47(9),738-745. |
MLA | Wang, T,et al."Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1 alpha expression".BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH 47.9(2014):738-745. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论