NPFF2 Receptor is Involved in the Modulatory Effects of Neuropeptide FF for Macrophage Cell Line

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	NPFF2 Receptor is Involved in the Modulatory Effects of Neuropeptide FF for Macrophage Cell Line
	Sun, YL; Sun, T; Zhang, XY; He, N; Zhuang, Y; Li, JY; Fang, Q; Wang, KR; Wang, R; Wang, R (reprint author), Lanzhou Univ, Sch Basic Med, 222 Tian Shui South Rd, Lanzhou 730000, Gansu, Peoples R China.
刊名	PROTEIN AND PEPTIDE LETTERS
	2014-05
卷号	21 期号:5 页码:490-502
关键词	Macrophage neuropeptide FF neuropeptide FF2 receptor NF-kappa B nitric oxide synthases
ISSN号	0929-8665
文献子类	Article
英文摘要	Neuropeptide FF (NPFF) interacts with specific receptors to regulate diverse biological processes. Its modulatory effect in the immune field, however, has not been fully explored yet. Here, we report that NPFF2 receptors may be functionally expressed in two immune cell models, the primary peritoneal macrophage and RAW 264.7 macrophage. Firstly, the mRNA levels of NPFF2 receptor were up-regulated in macrophages when treated with LPS for 24 to 72 h. Subsequently, our data hinted that NPFF regulates the viability of both kinds of macrophages. After treatment with RF9, a reported antagonist for both NPFF receptors, delayed or inhibited the NPFF-induced macrophages viability augmentation, suggesting the involvement of NPFF2 receptor. Furthermore, down-regulation of nitric oxide (NO) synthases (NOSs) partially significantly inhibited the viability augmentation of macrophages induced by NPFF, implying a nitric oxide synthases-dependent pathway is involved. However, the NOSs are not the only route by which NPFF affects the viability of macrophages. Pharmacological inhibitors of NF-B signal pathway also blocked the NPFF-induced macrophages growth, suggesting the involvement of the NF-B signal pathway. The regulation activity of NPFF for macrophages suggests that NPFF could act as a potential hormone in the control of immune system. Collectively, our data provide new evidence about the immune modulatory effect of NPFF, which will be helpful in extending the scope of NPFF functions.
学科主题	Biochemistry & Molecular Biology
出版地	SHARJAH
资助项目	国家自然科学基金项目 ; 国家科技重大专项 ; 长江学者和创新团队发展计划
项目编号	National Natural Science Foundation of China [91213302, 20932003, 21272102] ; Key National S&T Program "Major New Drug Development" of the Ministry of Science and Technology of China [2012ZX09504001-003] ; Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT: IRT1137]
语种	英语
WOS记录号	WOS:000331885600011
资助机构	NSFC ; MOST ; MOE
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121681]
专题	基础医学院_期刊论文
通讯作者	Wang, R (reprint author), Lanzhou Univ, Sch Basic Med, 222 Tian Shui South Rd, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Sun, YL,Sun, T,Zhang, XY,et al. NPFF2 Receptor is Involved in the Modulatory Effects of Neuropeptide FF for Macrophage Cell Line[J]. PROTEIN AND PEPTIDE LETTERS,2014,21(5):490-502.
APA	Sun, YL.,Sun, T.,Zhang, XY.,He, N.,Zhuang, Y.,...&Wang, R .(2014).NPFF2 Receptor is Involved in the Modulatory Effects of Neuropeptide FF for Macrophage Cell Line.PROTEIN AND PEPTIDE LETTERS,21(5),490-502.
MLA	Sun, YL,et al."NPFF2 Receptor is Involved in the Modulatory Effects of Neuropeptide FF for Macrophage Cell Line".PROTEIN AND PEPTIDE LETTERS 21.5(2014):490-502.