Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses

doi:10.1371/journal.pone.0013583

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	Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses
	Cheng, WX; Li, JS; Huang, CP; Yao, DP; Liu, N; Cui, SX; Jin, Y; Duan, ZJ; Cheng, WX (reprint author), China Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Beijing, Peoples R China.
刊名	PLOS ONE
	2010-10-25
卷号	5 期号:10 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0013583
文献子类	Article
英文摘要	The genus bocavirus includes bovine parvovirus (BPV), minute virus of canines (MVC), and a group of human bocaviruses (HBoV1-4). Using sequence-independent single primer amplification (SISPA), a novel bocavirus group was discovered with high prevalence (12.59%) in piglet stool samples. Two nearly full-length genome sequences were obtained, which were approximately 5,100 nucleotides in length. Multiple alignments revealed that they share 28.7-56.8% DNA sequence identity with other members of Parvovirinae. Phylogenetic analyses indicated their closest neighbors were members of the genus bocavirus. The new viruses had a putative non-structural NP1 protein, which was unique to bocaviruses. They were provisionally named porcine bocavirus 1 and 2 (PBoV1, PBoV2). PBoV1 and PBoV2 shared 94.2% nucleotide identity in NS1 gene sequence, suggesting that they represented two different bocavirus species. Two additional samples (6V, 7V) were amplified for 2,407 bp and 2,434 bp products, respectively, including a partial NP1 gene and the complete VP1 gene; Phylogenetic analysis indicated that 6Vand 7V grouped with PBoV1 and PBoV2 in the genus of bocavirus, but were in the separate clusters. Like other parvoviruses, PBoV1, PBoV2, 6Vand 7V also contained a putative secretory phospholipase A(2) (sPLA(2)) motif in the VP1 unique region, with a conserved HDXXY motif in the catalytic center. The conserved motif YXGXF of the Ca2+-binding loop of sPLA2 identified in human bocavirus was also found in porcine bocavirus, which differs from the YXGXG motif carried by most other parvoviruses. The observation of PBoV and potentially other new bocavirus genus members may aid in molecular and functional characterization of the genus bocavirus.
学科主题	Science & Technology - Other Topics
出版地	SAN FRANCISCO
资助项目	国家重点基础研究发展计划以及国家重大科学研究计划（973计划） ; 国家科技重大专项
项目编号	China Mega-Project for Infectious Disease [2009ZX10004-001] ; "973" National Key Basic Research Program of China [2007CB310500]
语种	英语
WOS记录号	WOS:000283422100007
资助机构	MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121464]
专题	基础医学院_期刊论文
通讯作者	Cheng, WX (reprint author), China Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Mol Virol & Genet Engn, Beijing, Peoples R China.
推荐引用方式 GB/T 7714	Cheng, WX,Li, JS,Huang, CP,et al. Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses[J]. PLOS ONE,2010,5(10):-.
APA	Cheng, WX.,Li, JS.,Huang, CP.,Yao, DP.,Liu, N.,...&Cheng, WX .(2010).Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses.PLOS ONE,5(10),-.
MLA	Cheng, WX,et al."Identification and Nearly Full-Length Genome Characterization of Novel Porcine Bocaviruses".PLOS ONE 5.10(2010):-.