Aberrant methylation of the THRB gene in tissue and plasma of breast cancer patients

doi:10.1016/j.cancergencyto.2009.09.010

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	Aberrant methylation of the THRB gene in tissue and plasma of breast cancer patients
	Ling, YQ; Xu, XY; Hao, J; Ling, XL; Du, XP; Liu, X; Zhao, XX; Liu, X (reprint author), Lanzhou Univ, Coll Basic Med, Dept Pathophysiol, Lanzhou 730020, Gansu, Peoples R China.
刊名	CANCER GENETICS AND CYTOGENETICS
	2010-01-15
卷号	196 期号:2 页码:140-145
ISSN号	0165-4608
DOI	10.1016/j.cancergencyto.2009.09.010
文献子类	Article
英文摘要	The thyroid hormone receptors (TR) have three major isoforms, TR alpha 1 TR alpha 2, and TR beta 1; these are ligand-dependent nuclear transcription factors. THRB, the gene encoding TR beta 1, is considered a potential cancer suppressor. The mechanism of its inactivation is not yet clear. Aberrant silencing of THRB in breast cancer tissue and plasma by promoter hypermethylation was investigated in the present study. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to examine THRB mRNA expression in the breast cancer tissues. Methylation-specific polymerase chain reaction (MSP) combined with nested PCR was used to determine the methylation status of the THRB gene promoter region in 40 cancer tissue and 40 plasma samples from breast cancer patients. Methylation status of MSP product in plasma was also evaluated by direct sequencing. The expression of THRB mRNA in breast cancer tissues was lower than that in the normal tissues; hypermethylation was found in 32 of 40 breast cancer tissues (80%) and in 28 of 40 plasma samples (70%). Loss of THRB gene expression was associated with the CpG island hypermethylation of promoter regions. THRB gene CpG island methylation was not related to clinical pathologic parameters. Sequencing results were identical to agarose gel electrophoresis results. The present results indicate that hypermethylation of THRB as an alternative gene silencing mechanism is highly prevalent in breast cancer. Methylated tumor-specific DNA may serve as a plasma biomarker for prognosis in patients with breast cancer. (C) 2010 Elsevier Inc. All rights reserved.
学科主题	Oncology ; Genetics & Heredity
出版地	NEW YORK
资助项目	中央高校基本科研业务费专项资金
项目编号	Gansu Provincial Science and Technology Department ; Medicine Foundation of Lanzhou University
语种	英语
WOS记录号	WOS:000277396000005
资助机构	LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121444]
专题	基础医学院_期刊论文
通讯作者	Liu, X (reprint author), Lanzhou Univ, Coll Basic Med, Dept Pathophysiol, Lanzhou 730020, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Ling, YQ,Xu, XY,Hao, J,et al. Aberrant methylation of the THRB gene in tissue and plasma of breast cancer patients[J]. CANCER GENETICS AND CYTOGENETICS,2010,196(2):140-145.
APA	Ling, YQ.,Xu, XY.,Hao, J.,Ling, XL.,Du, XP.,...&Liu, X .(2010).Aberrant methylation of the THRB gene in tissue and plasma of breast cancer patients.CANCER GENETICS AND CYTOGENETICS,196(2),140-145.
MLA	Ling, YQ,et al."Aberrant methylation of the THRB gene in tissue and plasma of breast cancer patients".CANCER GENETICS AND CYTOGENETICS 196.2(2010):140-145.