Chitosan microspheres enhance the immunogenicity of an A985B-based fusion protein containing multiple T-cell epitopes of Mycobacterium tuberculosis

doi:10.1016/j.ejpb.2006.11.028

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	Chitosan microspheres enhance the immunogenicity of an A985B-based fusion protein containing multiple T-cell epitopes of Mycobacterium tuberculosis
	Zhu, BD; Qie, YQ; Wang, JL; Zhang, Y; Wang, QZ; Xu, Y; Wang, HH; Wang, HH (reprint author), Fudan Univ, Sch Life Sci, Inst Genet, Handan Rd 220, Shanghai 200433, Peoples R China.
刊名	EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
	2007-06
卷号	66 期号:3 页码:318-326
关键词	Mycobacterium tuberculosis subunit vaccine fusion protein chitosan microspheres adjuvant
ISSN号	0939-6411
DOI	10.1016/j.ejpb.2006.11.028
文献子类	Article
英文摘要	To develop novel delivery system for tuberculosis (TB) subunit vaccine, biodegradable chitosan microspheres were prepared and used to deliver a fusion protein, Ag85B-MPT64(190-198)-Mtb8.4 (AMM for short), made from three Mycobacterium tuberculosis genes. AMM-loaded microspheres were first characterized for their morphology, size, zeta potential, loading efficiency, and in vitro release of AMM. C57BL/6 mice were immunized at weeks 1, 3 and 5 subcutaneously with AMM formulated in chitosan microspheres, in incomplete Freund's adjuvant (IFA), or in phosphate-buffered saline (PBS), respectively. Three weeks after the last immunization, humoral and cell-mediated immune responses were examined. It was shown that the microspheres bound AMM quite efficiently (loading efficiency: > 99%). AMM-loaded chitosan microspheres were observed as aggregated shapes with the average particle size of 5.78 +/- 0.65 mu m and zeta potential of 32.77 +/- 1.51 mV. In vitro release studies revealed that only small amount of antigen was released in 16 days. Following subcutaneous administration, splenocytes immunized with AMM in chitosan microspheres produced higher levels of IFN-gamma, compared to administration of AMM in PBS upon stimulation with Ag85B and synthetic peptide MPT64(190-198). The levels of Ag85B-specific IgG (H+L), IgG1 and IgG2a in sera of mice immunized with AMM in chitosan microspheres were also higher than those with AMM in PBS. These results indicate that chitosan microspheres when used as a carrier for fusion protein AMM could elicit strong humoral and cell-mediated immune responses. (c) 2007 Elsevier B.V. All rights reserved.
学科主题	Pharmacology & Pharmacy
出版地	AMSTERDAM
语种	英语
WOS记录号	WOS:000247542100002
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121369]
专题	基础医学院_期刊论文
通讯作者	Wang, HH (reprint author), Fudan Univ, Sch Life Sci, Inst Genet, Handan Rd 220, Shanghai 200433, Peoples R China.
推荐引用方式 GB/T 7714	Zhu, BD,Qie, YQ,Wang, JL,et al. Chitosan microspheres enhance the immunogenicity of an A985B-based fusion protein containing multiple T-cell epitopes of Mycobacterium tuberculosis[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2007,66(3):318-326.
APA	Zhu, BD.,Qie, YQ.,Wang, JL.,Zhang, Y.,Wang, QZ.,...&Wang, HH .(2007).Chitosan microspheres enhance the immunogenicity of an A985B-based fusion protein containing multiple T-cell epitopes of Mycobacterium tuberculosis.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,66(3),318-326.
MLA	Zhu, BD,et al."Chitosan microspheres enhance the immunogenicity of an A985B-based fusion protein containing multiple T-cell epitopes of Mycobacterium tuberculosis".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 66.3(2007):318-326.