Effect of rhubarb on contractile response of gallbladder smooth muscle strips isolated from guinea pigs

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	Effect of rhubarb on contractile response of gallbladder smooth muscle strips isolated from guinea pigs
	Luo, YL; Zeng, JW; Yu, M; Wei, YL; Qu, SY; Li, W; Zheng, TZ; Zheng, TZ (reprint author), Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China.
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2005-02-14
卷号	11 期号:6 页码:863-866
关键词	Rhubarb Gallbladder smooth muscle strips M receptor Ca2+ channel alpha receptor
ISSN号	1007-9327
文献子类	Article
英文摘要	AIM: To investigate the effect of rhubarb on contractile response of isolated gallbladder muscle strips from guinea pigs and its mechanism. METHODS: Guinea pigs were killed to remove the whole gallbladder. Two or three smooth muscle strips (8 mmx3 mm) were cut along the longitudinal direction. The mucosa on each strip was carefully removed. Each longitudinal muscle strip was suspended in a tissue chamber containing 5 mL Krebs solution (37 degrees C), bubbled continuously with 950 mL/L O-2 and 50 mL/L CO2. The resting tension (g), mean contractile amplitude (mm), and contractile frequency (waves/min) were simultaneously recorded on recorders. After 2-h equilibration, rhubarb (10, 20, 70, 200, 700, 1 000 g/L) was added cumulatively to the tissue chamber in turns every 2 min to observe their effects on gallbladder. Antagonists were given 3 min before administration of rhubarb to investigate the possible mechanism. RESULTS: Rhubarb increased the resting tension (from 0 to 0.40 +/- 0.02, P<0.001), and decreased the mean contractile amplitude (from 5.22 +/- 0.71 to 2.73 +/- 0.41, P<0.001). It also increased the contractile frequency of the gallbladder muscle strips in guinea pigs (from 4.09 +/- 0.46 to 6.08 +/- 0.35, P<0.001). The stimulation of rhubarb on the resting tension decreased from 3.98 +/- 0.22 to 1.58 +/- 0.12 by atropine (P<0.001), from 3.98 +/- 0.22 to 2.09 +/- 0.19 by verapamil (P<0.001) and from 3.98 +/- 0.22 to 2.67 +/- 0.43 by phentolamine (P<0.005). But the effect was not inhibited by hexamethonium (P>0.05). In addition, the action of mean amplitude and frequency was not inhibited by the above antagonists. CONCLUSION: Rhubarb can stimulate the motility of isolated gallbladder muscle strips from guinea pigs. The stimulation of rhubarb might be relevant with M receptor, Ca2+ channel and alpha receptor partly. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
学科主题	Gastroenterology & Hepatology
出版地	BEIJING
资助项目	甘肃省自然科学基金计划
项目编号	Key Laboratory of Pre-clinical Research for Chinese Herbs and New Drugs of Gansu Province ; Natural Scientific Foundation of Gansu Province [zs021-A25-059-Y]
语种	英语
WOS记录号	WOS:000208100300017
资助机构	GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121346]
专题	基础医学院_期刊论文
通讯作者	Zheng, TZ (reprint author), Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Luo, YL,Zeng, JW,Yu, M,et al. Effect of rhubarb on contractile response of gallbladder smooth muscle strips isolated from guinea pigs[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(6):863-866.
APA	Luo, YL.,Zeng, JW.,Yu, M.,Wei, YL.,Qu, SY.,...&Zheng, TZ .(2005).Effect of rhubarb on contractile response of gallbladder smooth muscle strips isolated from guinea pigs.WORLD JOURNAL OF GASTROENTEROLOGY,11(6),863-866.
MLA	Luo, YL,et al."Effect of rhubarb on contractile response of gallbladder smooth muscle strips isolated from guinea pigs".WORLD JOURNAL OF GASTROENTEROLOGY 11.6(2005):863-866.