Arsenic trioxide inhibits p-glycoprotein expression in multidrug-resistant human leukemia cells that overexpress the MDR1 gene

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	Arsenic trioxide inhibits p-glycoprotein expression in multidrug-resistant human leukemia cells that overexpress the MDR1 gene
	Wei, HL; Su, HX; Bai, DC; Zhao, HS; Ge, JG; Wang, B; Yao, XJ; Ma, LF; Wei, HL (reprint author), Lanzhou Med Coll, Lab Ctr Med Sci, Lanzhou 730000, Peoples R China.
刊名	CHINESE MEDICAL JOURNAL
	2003-11
卷号	116 期号:11 页码:1644-1648
关键词	arsenic trioxide leukemia multidrug resistance p-glycoprotein apoptosis
ISSN号	0366-6999
文献子类	Article
英文摘要	Objective To investigate the effects of arsenic trioxide (As(2)O(3)) on the apoptosis and p-glycoprotein (P-gp) expression of multidrug-resistant human leukemia cells. Methods Human multidrug-resistant leukemia cell line K562/ADM overexpressing the MDR1 gene, was used as the target cells. The cell proliferating activity was assessed using the MTT colorimetric assay. Cytomorphology was investigated under light, confocal and electron microscopes. DNA fragmentation was examined using agarose gel electrophoresis, while p-gp expression, cell cycle status and sub-G1 cells were determined using flow cytometry. Results Zero point five to 20 mumol/L AS(2)O(3) inhibited the proliferation of K562/ADM cells, and K562/ADM cells were more sensitive to As(2)O(3) than the parental K562 cells. As(2)O(3)-induced apoptosis of K562/ADM cells was determined by the observance of typical morphological changes and the appearance of DNA ladder and sub-G1 cell populations. As(2)O(3) significantly inhibited the P-gp expression of K562/ADM cells, and synergistically enhanced the sensitivity of the drug-resistant cells to adriamycin. Conclusions As(2)O(3) induces growth-inhibition and apoptosis, down-regulates P-gp expression and exerts a synergistic effect in combination with adriamycin in multidrug-resistant leukemia cells.
学科主题	General & Internal Medicine
出版地	BEIJING
语种	英语
WOS记录号	WOS:000187462900004
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121336]
专题	基础医学院_期刊论文
通讯作者	Wei, HL (reprint author), Lanzhou Med Coll, Lab Ctr Med Sci, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Wei, HL,Su, HX,Bai, DC,et al. Arsenic trioxide inhibits p-glycoprotein expression in multidrug-resistant human leukemia cells that overexpress the MDR1 gene[J]. CHINESE MEDICAL JOURNAL,2003,116(11):1644-1648.
APA	Wei, HL.,Su, HX.,Bai, DC.,Zhao, HS.,Ge, JG.,...&Wei, HL .(2003).Arsenic trioxide inhibits p-glycoprotein expression in multidrug-resistant human leukemia cells that overexpress the MDR1 gene.CHINESE MEDICAL JOURNAL,116(11),1644-1648.
MLA	Wei, HL,et al."Arsenic trioxide inhibits p-glycoprotein expression in multidrug-resistant human leukemia cells that overexpress the MDR1 gene".CHINESE MEDICAL JOURNAL 116.11(2003):1644-1648.