Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer

CORC > 中国农业科学院 > 北京畜牧兽医研究所

	Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer
	JU Hui-ming; BAI Li-jing; REN Hong-yan; MU Yu-lian; YANG Shu-lin; LI Kui
刊名	Agricultural Sciences in China
	2011
卷号	10 期号:3 页码:431-437
关键词	type-A spermatogonia transgenic mice enhanced green fluorescent protein type-A spermatogonia-mediated gene transfer
ISSN号	1671-2927
英文摘要	Type-A spermatogonia first appear at between 3-7 d postnatally in mice and are the only immortalized diploid cells that reproduce in adulthood in these animals.In our current study,we explored the feasibility of producing stable transgenic mice using these cells.Enhanced pEGFP-N1 plasmids were suspended in ExGen500 transfection reagent and injected at different angles into the testes of 7-d-old male ICR mice.The resulting type-A spermatogonia-mediated gene transfer (TASMGT)mice were then mated with normal females at different stages of sexual maturity(6,12,and 24 wk).The integration and expression of the introduced EGFP gene was evaluated in the F_1transgenic offspring by PCR and Southern blotting analysis.The foreign gene integration rates for a low-dose group(15μL gene suspension injected into each testis)and a high-dose group(30μL suspensions injected)at the three stages of female sexual maturity tested were 11.76%(2/17),14.29%(3/21),and 11.11%(2/18),and 5%(1/20),5.56%(1/18),and 0(0/17),respectively.The average integration rates for these two dose groups were 12.5%(7/56)and 3.64%(2/55),respectively,which was a significant difference(P<0.05).Semi-quantitative RT-PCR analysis further showed that the introduced GFP gene was expressed in 3/9 integration mice.In addition,GFP expression was observed in the sperm cells from the TASMGT mice,and also in the embryos and F_2pups from the F_1generation transgenic mice.Hence,although the foreign gene integration rate for TASMGT is not high and the transgenic offspring show as yet unexplained defects,our results indicate that this method is a potentially feasible and reproducible new approach to creating transgenic mice.
学科主题	植物保护
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/119662]
专题	北京畜牧兽医研究所
作者单位	Institute of Animal Science,Chinese Academy of Agricultural Sciences, Key Laboratory for Farm Animal Genetic Resources and Utilization,Ministry of Agriculture, Beijing, 100193
推荐引用方式 GB/T 7714	JU Hui-ming,BAI Li-jing,REN Hong-yan,et al. Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer[J]. Agricultural Sciences in China,2011,10(3):431-437.
APA	JU Hui-ming,BAI Li-jing,REN Hong-yan,MU Yu-lian,YANG Shu-lin,&LI Kui.(2011).Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer.Agricultural Sciences in China,10(3),431-437.
MLA	JU Hui-ming,et al."Production of Transgenic Mice by Type-A Spermatogonia-Mediated Gene Transfer".Agricultural Sciences in China 10.3(2011):431-437.