Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography

doi:10.1016/j.seppur.2010.02.012

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	Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography
	Xie, Qianqian 1; Wei, Yun 1; Zhang, Guoliang 2
刊名	SEPARATION AND PURIFICATION TECHNOLOGY
	2010
卷号	72 期号:2 页码:229-233
关键词	High-speed counter-current chromatography Flaveria bidentis (L.) Kuntze Patuletin-3-O-glucoside Hyperoside Astragalin
ISSN号	1383-5866
DOI	10.1016/j.seppur.2010.02.012
通讯作者	Xie, Qianqian
英文摘要	In order to utilize and control the invasive weed, Flaveria bidentis (L,) Kuntze, bioactive compounds mainly flavonoids from F. bidentis (L.) Kuntze were studied. High-speed counter-current chromatography (HSCCC) was successfully used for the separation of flavonol glycosides from F. bidentis (L.) Kuntze. The two-phase solvent system composed of ethyl acetate-methanol-water (10:0.4:10, v/v) was used for HSCCC. About 400 mg of the crude extract was separated by HSCCC, yielding 3.6 mg of patuletin-3-O-glucoside at a purity of over 97%; 4.4 mg of astragalin (kaempferol-3-O-glucoside) at a purity of over 98% and 4.5 mg of a mixture of hyperoside (quercetin-3-O-galactoside) and 6-methoxykaempferol-3-O-galactoside constituting over 97% of the fraction. The chemical structures were confirmed by MS and (1)H, (13)C, 1-D TOCSY NMR. (C) 2010 Elsevier B.V. All rights reserved.
学科主题	Engineering, Chemical ; ENGINEERING, CHEMICAL
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000277640700015
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19951]
专题	农业环境与可持续发展研究所_环境修复研究室
作者单位	1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China 2.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xie, Qianqian,Wei, Yun,Zhang, Guoliang. Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2010,72(2):229-233.
APA	Xie, Qianqian,Wei, Yun,&Zhang, Guoliang.(2010).Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography.SEPARATION AND PURIFICATION TECHNOLOGY,72(2),229-233.
MLA	Xie, Qianqian,et al."Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography".SEPARATION AND PURIFICATION TECHNOLOGY 72.2(2010):229-233.