A Universal DNA-Based Protein Detection System

doi:10.1021/ja405872g

CORC > 中国农业科学院 > 农业环境与可持续发展研究所 > 环境工程研究室

	A Universal DNA-Based Protein Detection System
	Tran, Thua N. N.1; Cui, Jinhui 1,4; Hartman, Mark R.1; Peng, Songming 1; Funabashi, Hisakage 1,5; Duan, Faping 1; Yang, Dayong 1; March, John C.1; Lis, John T.2; Cui, Haixin 4
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2013
卷号	135 期号:38 页码:14008-14011
ISSN号	0002-7863
DOI	10.1021/ja405872g
通讯作者	Tran, Thua N. N.
英文摘要	Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.
学科主题	Chemistry, Multidisciplinary ; CHEMISTRY, MULTIDISCIPLINARY
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000330162900003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19837]
专题	农业环境与可持续发展研究所_环境工程研究室
作者单位	1.Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA 2.Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA 3.Cornell Univ, Kavli Inst Cornell Nanoscale Sci, Ithaca, NY 14853 USA 4.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China 5.Hiroshima Univ, Inst Sustainable Sci & Dev, Higashihiroshima 7398527, Japan
推荐引用方式 GB/T 7714	Tran, Thua N. N.,Cui, Jinhui,Hartman, Mark R.,et al. A Universal DNA-Based Protein Detection System[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(38):14008-14011.
APA	Tran, Thua N. N..,Cui, Jinhui.,Hartman, Mark R..,Peng, Songming.,Funabashi, Hisakage.,...&Luo, Dan.(2013).A Universal DNA-Based Protein Detection System.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(38),14008-14011.
MLA	Tran, Thua N. N.,et al."A Universal DNA-Based Protein Detection System".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.38(2013):14008-14011.