基于混合固定相色谱柱的SPE-HPLC法检测环境水体中三嗪类除草剂

	基于混合固定相色谱柱的SPE-HPLC法检测环境水体中三嗪类除草剂
	王硕蕾 1; 王宛 2; 范中南 1; 孙宝利 1; 黄金丽 1; 董一威 1; 陕红 1; 汪群杰 1
刊名	分析试验室
	2011
卷号	30 期号:7 页码:98-102
关键词	固相萃取高效液相色谱三嗪类除草剂混合固定相色谱柱
ISSN号	1000-0720
其他题名	SPE-HPLC analysis of triazine herbicides in environmental waters using mixed stationary phase
英文摘要	利用混合固定相色谱柱(Optim ix SCX/C8)分析了8种三嗪类化合物,在0.01 mol/L乙酸钠缓冲溶液(pH4.2)-CH3CN(75:25,V/V)等度洗脱的流动相条件下,实现了利用液相色谱方法分离同分异构体敌草净和西草净,并对比了相同色谱条件下8种目标物在C8色谱柱上的分离效果;比较了PEP和C18固相萃取柱在处理大体积水样时对目标物的吸附性能,最终选用PEP柱净化富集,建立了8种三嗪类化合物的SPE-HPLC分析方法。结果显示,8种三嗪类化合物在0.5～50 mg/L范围内线性良好,相关系数优于0.9992;在1～50μg/L范围内,方法的平均加标回收率在79.2%～103.1%之间,RSD为2.2%～7.3%;检出限(LOD)及定量限(LOQ)分别为0.08～0.24μg/L和0.2～0.8μg/L。可用于环境水样中多种三嗪类除草剂残留的检测。; A method of SPE-HPLC for the separation of eight triazine herbicides is described in this paper.A pair of isomers（Desmetryn and Simetryn） which could hardly be resolved by conventional reversed-phase were originally separated with good resolution.The separation was performed on a novel mixed stationary phase Optimix SCX/C8（250 mm × 4.6 mm,i.d.5 μm） using a mixture of 0.01 mol/L sodium acetate buffer（pH 4.2）-CH3CN（75:25,V/V） as the mobile phase.A comparison with a conventional reversed-phase column for the separation of the 8 triazines was also conducted.The method for solid phase extraction was studied for details.Two SPE cartridges were compared in adsorption performance when handling large amounts of water samples.The PEP cartridge was selected for further analysis.The proposed method was validated with respect to linearity,limits of detection and repeatabilities,and satisfactory results were obtained.The linear ranges for 8 triazines were 0.550 mg/L with correlation coefficients above 0.9992.In the range of 150 μg/L,the average recoveries in spiked water samples ranged from 79.2% to 103.1% with RSDs（%） between 2.2% and 7.3%.The LODs and LOQs of the method were 0.080.24 μg/L and 0.20.8 μg/L,respectively.The method for quality control purposes can be applied to the simultaneous determination of multi-residues of triazine herbicides in surface water as well as sauce water for drinking use.
学科主题	化学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17168]
专题	农业环境与可持续发展研究所_职能部门
作者单位	1.中国农业科学院农业环境与可持续发展研究所, 北京, 100081 2.天津博纳艾杰尔科技有限公司, 天津, 300457
推荐引用方式 GB/T 7714	王硕蕾,王宛,范中南,等. 基于混合固定相色谱柱的SPE-HPLC法检测环境水体中三嗪类除草剂[J]. 分析试验室,2011,30(7):98-102.
APA	王硕蕾.,王宛.,范中南.,孙宝利.,黄金丽.,...&汪群杰.(2011).基于混合固定相色谱柱的SPE-HPLC法检测环境水体中三嗪类除草剂.分析试验室,30(7),98-102.
MLA	王硕蕾,et al."基于混合固定相色谱柱的SPE-HPLC法检测环境水体中三嗪类除草剂".分析试验室 30.7(2011):98-102.