Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies

doi:10.3390/ijms17111881

	Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies
	Xu, Leifeng 1; Yang, Panpan 1,2; Yuan, Suxia 1; Feng, Yayan 1; Xu, Hua 1; Cao, Yuwei 1; Ming, Jun 1
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
卷号	17 期号:11 页码:-
关键词	Lilium spp. anthocyanins purple ovaries transcriptome transcriptional network
ISSN号	1422-0067
DOI	10.3390/ijms17111881
通讯作者	Xu, Leifeng
英文摘要	Lily tepals have a short lifespan. Once the tepals senesce, the ornamental value of the flower is lost. Some cultivars have attractive purple ovaries and fruits which greatly enhance the ornamental value of Asiatic hybrid lilies. However, little is known about the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries. To investigate the transcriptional network that governs purple ovary coloration in Asiatic hybrid lilies, we obtained transcriptome data from green ovaries (S1) and purple ovaries (S2) of Asiatic "Tiny Padhye". Comparative transcriptome analysis revealed 4228 differentially expressed genes. Differential expression analysis revealed that ten unigenes including four CHS genes, one CHI gene, one F3H gene, one F3'H gene, one DFR gene, one UFGT gene, and one 3RT gene were significantly up-regulated in purple ovaries. One MYB gene, LhMYB12-Lat, was identified as a key transcription factor determining the distribution of anthocyanins in Asiatic hybrid lily ovaries. Further qPCR results showed unigenes related to anthocyanin biosynthesis were highly expressed in purple ovaries of three purple-ovaried Asiatic hybrid lilies at stages 2 and 3, while they showed an extremely low level of expression in ovaries of three green-ovaried Asiatic hybrid lilies during all developmental stages. In addition, shading treatment significantly decreased pigment accumulation by suppressing the expression of several unigenes related to anthocyanin biosynthesis in ovaries of Asiatic "Tiny Padhye". Lastly, a total of 15,048 Simple Sequence Repeats (SSRs) were identified in 13,710 sequences, and primer pairs for SSRs were designed. The results could further our understanding of the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries.
学科主题	Chemistry, Multidisciplinary
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000388809600114
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105893]
专题	蔬菜花卉研究所_花卉研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Nanjing Forestry Univ, Coll Landscape Architecture, Nanjing 210037, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Xu, Leifeng,Yang, Panpan,Yuan, Suxia,et al. Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(11):-.
APA	Xu, Leifeng.,Yang, Panpan.,Yuan, Suxia.,Feng, Yayan.,Xu, Hua.,...&Ming, Jun.(2016).Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(11),-.
MLA	Xu, Leifeng,et al."Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.11(2016):-.