A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei

doi:10.1186/s40064-016-2320-4

	A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei
	Li, Yan-Lin 1,2; Huang, San-Wen 1,3; Zhang, Jia-Yin 4; Bu, Feng-Jiao 3; Lin, Tao 3; Zhang, Zhong-Hua 3; Xiong, Xing-Yao 1,3
刊名	SPRINGERPLUS
	2016
卷号	5 页码:-
关键词	Taxus Haploid Endosperm Callus initiation Homozygote SNP mark
ISSN号	2193-1801
DOI	10.1186/s40064-016-2320-4
通讯作者	Li, Yan-Lin
英文摘要	Obtainment and characterization of the novel endosperm callus of Taxus chinensis Rehd. var. mairei are valuable for haploid breeding, genome, and functional genome in Taxus. Callus was obtained by hydropriming with sterile water for 3 days and suitable medium composition. The highest callus induction (70.89 %) and lower browning ratio (7.95 %) were obtained from Gamborg (B-5) medium supplemented with 30 g l(-1) of sucrose, 2.5 mg l(-1) of 2,4-dichlorophenoxyacetic (2,4-D), 0.5 mg l(-1) of 6-benzylademine (6-BA) and 7 g l(-1) of agar under dark conditions. The auxin of 2,4-D had a better efficiency of callus induction than naphthylacetic acid, and over 1 mg l(-1) of 6-BA was inhibitory to the callogensis of endosperm. The endosperm callus was haploid which was detectable by the flow cytometry. The genome block of homozygosity of callus was homozygous which was indicated by PCR-based SNP marks. The homozygous haploid of endosperm callus in vitro culture may be useful tools for taxoid-metabolism of gene engineering and bio-fermentation engineering.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	SPRINGER INTERNATIONAL PUBLISHING AG
WOS记录号	WOS:000377612900001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100814]
专题	蔬菜花卉研究所_马铃薯研究室
作者单位	1.Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China 2.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 4.State Key Lab Subhlth Intervent Technol, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Li, Yan-Lin,Huang, San-Wen,Zhang, Jia-Yin,et al. A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei[J]. SPRINGERPLUS,2016,5:-.
APA	Li, Yan-Lin.,Huang, San-Wen.,Zhang, Jia-Yin.,Bu, Feng-Jiao.,Lin, Tao.,...&Xiong, Xing-Yao.(2016).A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei.SPRINGERPLUS,5,-.
MLA	Li, Yan-Lin,et al."A protocol of homozygous haploid callus induction from endosperm of Taxus chinensis Rehd. var. mairei".SPRINGERPLUS 5(2016):-.